MetaCyc Pathway: pyruvate fermentation to butanol II (engineered) in Pseudomonas simiae WCS417

Reactions and Genes	L-Isoleucine (C)
Acetyl-CoA C-acyltransferase: 2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PS417_06710	-0.9
PS417_07580	N.D.
PS417_10515	-0.2
PS417_13855	-4.5
PS417_15175	N.D.
PS417_19210	-0.1
PS417_21220	+0.3
PS417_03145	-0.3
3-hydroxyacyl-CoA dehydrogenase (in reverse): acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PS417_07575	+0.3
PS417_21215	-0.0
PS417_26285	-0.1
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
PS417_07030	+0.2
PS417_07575	+0.3
PS417_08535	+0.3
PS417_10680	+0.1
PS417_11445	-0.8
PS417_22625	+0.1
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse): crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
PS417_09185	-0.0
PS417_10685	-0.2
PS417_13840	-0.4
PS417_13850	-5.7
PS417_14420	-0.2
PS417_15190	+0.1
PS417_16045	-0.1
PS417_20795	+0.1
PS417_04135	+0.1
Butanal dehydrogenase (in reverse): butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information