MetaCyc Pathway: fatty acid β-oxidation VI (mammalian peroxisome) in Pseudomonas putida KT2440

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Reactions and Genes 2-Methylbutanoic (C)
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a 2,3,4-saturated fatty acid + ATP + coenzyme A→a 2,3,4-saturated fatty acyl CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
PP_1412 -0.4
PP_1845 -0.3
PP_2047 +0.0
PP_2136 -1.8
PP_2217 -5.2
PP_3283 -0.2
PP_3284 -0.1
PP_3358 +0.2
PP_3491 -1.5
PP_3726 +0.4
PP_3732 -0.1
PP_4030 +0.0
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3R)-3-hydroxyacyl-CoA (EC 4.2.1.119)
PP_4552 N.D.
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
PP_2047 +0.0
PP_2136 -1.8
a 2,3,4-saturated (3R)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.M19)
No genes
Acetyl-CoA C-acyltransferase (in reverse):
a 2,3,4-saturated 3-oxoacyl-CoA + coenzyme A→a 2,3,4-saturated fatty acyl CoA + acetyl-CoA
(EC 2.3.1.16)
PP_0582 +0.0
PP_1377 -0.0
PP_2051 +0.1
PP_2137 -1.4
PP_2215 -5.3
PP_3280 -0.5
PP_3355 +0.0
PP_3754 -5.1
PP_4636 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information