MetaCyc Pathway: L-isoleucine degradation I in Pseudomonas putida KT2440

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Reactions and Genes 2-Methylbutanoic (C)
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
PP_3511 -5.4
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
PP_3492 -0.1
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
PP_1412 -0.4
PP_2047 +0.0
PP_2136 -1.8
PP_3726 +0.4
PP_3732 -0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
PP_2047 +0.0
PP_2136 -1.8
PP_2214 -3.5
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PP_0582 +0.0
PP_1377 -0.0
PP_2051 +0.1
PP_2137 -1.4
PP_2215 -5.3
PP_3280 -0.5
PP_3355 +0.0
PP_3754 -5.1
PP_4636 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information