MetaCyc Pathway: pyruvate fermentation to butanoate in Pseudomonas putida KT2440

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Reactions and Genes 2-Methylbutanoic (C)
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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PP_0582 -0.0
PP_1377 -0.2
PP_2051 +0.0
PP_2137 -1.2
PP_2215 -5.2
PP_3280 -0.1
PP_3355 +0.2
PP_3754 -3.7
PP_4636 +0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PP_2047 -0.0
PP_2136 -1.6
PP_3755 -2.6
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
PP_1412 -0.3
PP_2047 -0.0
PP_2136 -1.6
PP_3726 -0.6
PP_3732 -0.1
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Phosphate butyryltransferase:
butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)
No genes
Branched-chain-fatty-acid kinase (in reverse):
butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information