MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Pseudomonas putida KT2440

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Reactions and Genes 1,3-Hexanediol (C)
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Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
PP_3492 +0.1
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
PP_0368 -3.6
PP_0370 +0.1
PP_2048 +1.0
PP_3354 +0.1
PP_3554 +0.1
PP_4780 -0.2
PP_4948 +0.4
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PP_1412 -0.0
PP_1845 -0.4
PP_2047 +7.1
PP_2136 -2.9
PP_2217 -2.9
PP_3283 -0.3
PP_3284 +0.1
PP_3358 -0.0
PP_3491 -0.2
PP_3726 +0.6
PP_3732 +0.1
PP_4030 -0.2
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PP_1412 -0.0
PP_1845 -0.4
PP_2047 +7.1
PP_2136 -2.9
PP_2217 -2.9
PP_3283 -0.3
PP_3284 +0.1
PP_3358 -0.0
PP_3491 -0.2
PP_3726 +0.6
PP_3732 +0.1
PP_4030 -0.2
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PP_1412 -0.0
PP_1845 -0.4
PP_2047 +7.1
PP_2136 -2.9
PP_2217 -2.9
PP_3283 -0.3
PP_3284 +0.1
PP_3358 -0.0
PP_3491 -0.2
PP_3726 +0.6
PP_3732 +0.1
PP_4030 -0.2
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
PP_2047 +7.1
PP_2136 -2.9
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
No genes
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
PP_2047 +7.1
PP_2136 -2.9
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PP_0582 +0.1
PP_1377 -0.1
PP_2051 -0.1
PP_2137 -3.7
PP_2215 +0.2
PP_3280 +0.3
PP_3355 +0.0
PP_3754 -0.3
PP_4636 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PP_0582 +0.1
PP_1377 -0.1
PP_2051 -0.1
PP_2137 -3.7
PP_2215 +0.2
PP_3280 +0.3
PP_3355 +0.0
PP_3754 -0.3
PP_4636 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PP_0582 +0.1
PP_1377 -0.1
PP_2051 -0.1
PP_2137 -3.7
PP_2215 +0.2
PP_3280 +0.3
PP_3355 +0.0
PP_3754 -0.3
PP_4636 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information