MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Pseudomonas putida KT2440

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Reactions and Genes D-2-Aminobutyric (N)
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Glutamate dehydrogenase (NAD(P)(+)) (in reverse):
2-oxoglutarate + ammonium + NAD(P)H + H+→L-glutamate + NAD(P)+ + H2O (EC 1.4.1.3)
No genes
Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)
PP_0269 +0.8
PP_1060 +0.6
Glutamate synthase (NADH) (in reverse):
2-oxoglutarate + L-glutamine + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
PP_4037 +0.5
PP_5075 -5.2
PP_5076 -5.4
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
PP_0269 +0.8
PP_0417 -5.3
PP_0420 -5.2
PP_0511 +0.3
PP_0613 +0.1
PP_0727 -0.4
PP_0930 N.D.
PP_0931 N.D.
PP_0932 N.D.
PP_0991 +0.6
PP_1032 N.D.
PP_1060 +0.6
PP_1374 -1.2
PP_1460 +0.5
PP_1610 N.D.
PP_1691 -0.1
PP_1750 +0.2
PP_1842 -0.5
PP_2323 -0.3
PP_2329 -0.6
PP_2447 -0.3
PP_2453 +0.2
PP_3464 +0.2
PP_3598 +0.2
PP_4037 +0.5
PP_4723 -10.7
PP_4724 -10.8
PP_5075 -5.2
PP_5076 -5.4
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
PP_2178 +0.6
PP_3148 -0.2
PP_4399 -0.8
PP_4547 -0.5
PP_5046 N.D.
PP_5183 -2.8
PP_5184 +1.3
PP_5299 -0.7
Glutamate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + ammonium + NADPH + H+→L-glutamate + NADP+ + H2O (EC 1.4.1.4)
PP_0675 -0.3
PP_5075 -5.2
Glutamate synthase (NADPH) (in reverse):
2-oxoglutarate + ammonia + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
PP_0511 +0.3
PP_0991 +0.6
PP_1374 -1.2
PP_1460 +0.5
PP_1691 -0.1
PP_2323 -0.3
PP_2447 -0.3
PP_3464 +0.2
PP_4037 +0.5
PP_5075 -5.2
PP_5076 -5.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information