MetaCyc Pathway: superpathway of L-threonine biosynthesis in Pseudomonas putida KT2440

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Reactions and Genes R2A control with 0.2% methanol
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
PP_0486 -0.1
PP_0858 -0.1
PP_1109 +0.7
PP_1872 -0.1
PP_2642 +0.2
PP_2948 -0.0
PP_3544 -0.1
PP_3750 -0.1
PP_3786 +0.1
PP_4197 +1.3
PP_5275 -0.2
PP_5342 -0.0
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
PP_4473 N.D.
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
PP_1147 -0.3
PP_1989 N.D.
PP_1992 +0.3
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
PP_0664 +0.0
PP_1470 N.D.
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
PP_0121 N.D.
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
PP_0662 -0.1
PP_1471 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information