MetaCyc Pathway: superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass in Escherichia coli BW25113

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Reactions and Genes Phage_N4_cage_4_mice _Feces_40 hr
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D-glucopyranose[out] + an [HPr protein]-Nπ-phospho-L-histidine[in]→D-glucopyranose 6-phosphate[in] + an [HPr]-L-histidine[in] (EC 2.7.1.199)
b1101 (ptsG) -0.8
b1817 (manX) -0.7
b1818 (manY) -0.9
b1819 (manZ) -0.9
b2417 (crr) N.D.
b3722 (bglF) -0.0
Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
b2843 (kduI) -0.1
b4025 (pgi) -1.7
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
b1723 (pfkB) +0.1
b3916 (pfkA) -2.3
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
b2097 (b2097) +0.0
b2925 (fbaA) N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
b0383 (phoA) +0.0
b0766 (ybhA) +0.1
b2930 (yggF) +0.2
b3925 (glpX) +0.1
b4232 (fbp) +0.1
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
b3919 (tpiA) N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA) N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk) N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA) +0.2
b3612 (gpmI) -1.4
b4395 (ytjC) -0.2
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
b0755 (gpmA) +0.2
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno) N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF) -0.5
b1854 (pykA) +0.6
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
b1702 (pps) -0.1
pyruvate + coenzyme A + NAD+→acetyl-CoA + CO2 + NADH (EC 1.2.1.104)
b0114 (aceE) -2.6
b0115 (aceF) N.D.
b0116 (lpd) N.D.
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
b0720 (gltA) -0.3
Malate synthase:
acetyl-CoA + glyoxylate + H2O→(S)-malate + coenzyme A + H+ (EC 2.3.3.9)
b2976 (glcB) +0.0
b4014 (aceB) +0.3
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
b0118 (acnB) +0.4
b0771 (ybhJ) +0.1
b1276 (acnA) +0.2
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
b0118 (acnB) +0.4
b0771 (ybhJ) +0.1
b1276 (acnA) +0.2
Isocitrate lyase:
D-threo-isocitrate→glyoxylate + succinate (EC 4.1.3.1)
b4015 (aceA) -0.0
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
b1136 (icd) N.D.
2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
b0116 (lpd) N.D.
Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
b0728 (sucC) -0.3
b0729 (sucD) -0.5
Succinate dehydrogenase (ubiquinone):
succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse):
fumarate + H2O→(S)-malate (EC 4.2.1.2)
b1611 (fumC) -0.1
b1612 (fumA) +0.0
b1675 (ydhZ) +0.1
b2929 (yggD) -0.0
b4122 (fumB) -0.0
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
b0801 (ybiC) +0.2
b1479 (sfcA) +0.0
b3236 (mdh) -0.3
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
b2210 (mqo) +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information