MetaCyc Pathway: 3-methyl-branched fatty acid α-oxidation in Pseudomonas simiae WCS417

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Reactions and Genes Fraxetin 3 mM; liquid stress
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Long-chain-fatty-acid--CoA ligase:
a 3-methyl-branched 2,3,4-saturated fatty acid + ATP + coenzyme A→a 3-methyl-branched 2,3,4-saturated fatty acyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
PS417_15170 -0.1
PS417_17010 +0.0
PS417_19290 -0.0
PS417_22065 -1.3
PS417_22070 -0.1
a 3-methyl-branched 2,3,4-saturated fatty acyl-CoA + 2-oxoglutarate + dioxygen→a 2-hydroxy-3-methyl-branched 2,3,4-saturated fatty acyl-CoA + CO2 + succinate
No genes
a 2-hydroxy-3-methyl-branched 2,3,4-saturated fatty acyl-CoA→a 2-methyl branched 2,3,4-saturated fatty aldehyde + formyl-CoA (EC 4.1.2.63)
No genes
Formyl-CoA hydrolase:
formyl-CoA + H2O→formate + coenzyme A + H+
(EC 3.1.2.10)
No genes
Aldehyde dehydrogenase (NAD(+)):
a 2-methyl branched 2,3,4-saturated fatty aldehyde + NAD+ + H2O→a 2-methyl branched 2,3,4-saturated fatty acid + NADH + 2 H+
(EC 1.2.1.3)
PS417_07290 +0.2
PS417_11170 +0.1
PS417_14675 +0.2
PS417_17430 -0.4
PS417_18205 +0.3
PS417_24810 +0.1
PS417_02645 -0.1
PS417_26845 -0.0
PS417_27745 +0.5
PS417_04200 -0.1
Long-chain-fatty-acid--CoA ligase:
a 2-methyl branched 2,3,4-saturated fatty acid + ATP + coenzyme A→a 2-methyl branched 2,3,4-saturated fatty acyl-CoA + AMP + diphosphate
(EC 6.2.1.3)
PS417_15170 -0.1
PS417_17010 +0.0
PS417_19290 -0.0
PS417_22065 -1.3
PS417_22070 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information