MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Pseudomonas simiae WCS417

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Reactions and Genes 80% Methanol 3 mM; solid stress
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
PS417_05250 -0.0
PS417_05415 N.D.
PS417_06300 N.D.
PS417_06580 -0.2
PS417_06685 -0.1
PS417_08425 -0.0
PS417_11435 -0.0
PS417_15505 +0.4
PS417_16695 -0.2
PS417_17275 +0.1
PS417_17325 +0.1
PS417_17690 +0.1
PS417_17915 +0.2
PS417_18410 -0.5
PS417_19230 -0.1
PS417_19985 N.D.
PS417_02000 -0.1
PS417_02005 -0.0
PS417_21105 -1.4
PS417_21210 +0.2
PS417_21855 -0.5
PS417_22155 +0.1
PS417_22930 N.D.
PS417_23070 N.D.
PS417_24040 N.D.
PS417_24045 N.D.
PS417_25280 -0.1
PS417_25720 -1.4
PS417_25725 -0.4
PS417_28355 -0.3
PS417_04235 N.D.
PS417_04240 N.D.
PS417_04245 N.D.
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate (EC 6.1.1.23)
PS417_22465 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP (EC 6.3.5.6)
PS417_05250 -0.0
PS417_17690 +0.1
PS417_04235 N.D.
PS417_04240 N.D.
PS417_04245 N.D.
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate (EC 6.3.5.6)
PS417_05250 -0.0
PS417_17690 +0.1
PS417_04235 N.D.
PS417_04240 N.D.
PS417_04245 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information