MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Escherichia coli ECOR27

Add experiment(s):


Reactions and Genes Sodium-DL-Lactate
remove
2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
NOLOHH_03060 -4.8
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
NOLOHH_03070 (leuC1) -7.0
NOLOHH_03075 (leuD) -5.7
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
NOLOHH_03070 (leuC1) -7.0
NOLOHH_03075 (leuD) -5.7
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
NOLOHH_03065 (leuA) -7.4
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
NOLOHH_03065 (leuA) -7.4
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
NOLOHH_01650 (frmA) -0.1
NOLOHH_01805 (yahK) -0.2
NOLOHH_04010 (ahr) -0.1
NOLOHH_07750 (yiaY) +0.1
NOLOHH_09500 (acuI) +0.1
NOLOHH_19040 (adhP) -0.4
NOLOHH_20445 (adhE) -0.1

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information