MetaCyc Pathway: L-arginine biosynthesis I (via L-ornithine) in Escherichia coli ECOR27

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Reactions and Genes L-Rhamnose
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Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
hydrogen carbonate + 2 ATP + L-glutamine + H2O→carbamoyl phosphate + L-glutamate + 2 ADP + 2 H+ + phosphate (EC 6.3.5.5)
NOLOHH_03270 (carB) -4.9
NOLOHH_03275 (carA) -5.7
Amino-acid N-acetyltransferase:
L-glutamate + acetyl-CoA→N-acetyl-L-glutamate + coenzyme A + H+ (EC 2.3.1.1)
NOLOHH_11945 (argA) -4.3
Acetylglutamate kinase:
N-acetyl-L-glutamate + ATP→N-acetylglutamyl-phosphate + ADP (EC 2.7.2.8)
NOLOHH_05725 -5.6
N-acetyl-gamma-glutamyl-phosphate reductase (in reverse):
N-acetylglutamyl-phosphate + NADPH + H+→N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.38)
NOLOHH_05730 (argC) -5.3
Acetylornithine transaminase (in reverse):
N-acetyl-L-glutamate 5-semialdehyde + L-glutamate→N-acetyl-L-ornithine + 2-oxoglutarate (EC 2.6.1.11)
NOLOHH_09030 (argD,dapC) -0.2
NOLOHH_12735 (gabT) +0.0
NOLOHH_17420 (astC) -0.2
Acetylornithine deacetylase:
N-acetyl-L-ornithine + H2O→L-ornithine + acetate (EC 3.5.1.16)
NOLOHH_05735 (argE) -4.9
Ornithine carbamoyltransferase:
carbamoyl phosphate + L-ornithine→L-citrulline + H+ + phosphate (EC 2.1.3.3)
NOLOHH_04090 (argF) -4.4
Argininosuccinate synthase:
L-citrulline + L-aspartate + ATP→L-arginino-succinate + AMP + diphosphate + H+ (EC 6.3.4.5)
NOLOHH_09890 (argG) -5.8
Argininosuccinate lyase:
L-arginino-succinate→L-arginine + fumarate (EC 4.3.2.1)
NOLOHH_05720 (argH) -6.7

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information