MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Escherichia coli ECOR27

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Reactions and Genes L-Arabinose
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
NOLOHH_03040 (ilvN) -0.4
NOLOHH_03045 (ilvI) +0.2
NOLOHH_06785 -0.3
NOLOHH_06790 (ilvG) -2.0
NOLOHH_07325 -0.2
NOLOHH_07330 (ilvN) -0.4
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
NOLOHH_06760 (ilvC) -2.9
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
NOLOHH_06775 (ilvD) -2.3
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
NOLOHH_01650 (frmA) -0.4
NOLOHH_01805 (yahK) -0.3
NOLOHH_04010 (ahr) -0.2
NOLOHH_07750 (yiaY) -0.1
NOLOHH_09500 (acuI) +0.1
NOLOHH_19040 (adhP) -0.9
NOLOHH_20445 (adhE) +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information