MetaCyc Pathway: L-isoleucine degradation I in Hydrogenophaga sp. GW460-11-11-14-LB1

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Reactions and Genes Ying_Others16 rep A; time point 3
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Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
GFF1835 +0.7
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
GFF1003 -0.3
GFF1566 -0.3
GFF2070 N.D.
GFF2072 +0.1
GFF2109 -0.1
GFF2111 +0.0
GFF238 N.D.
GFF2746 -0.0
GFF2842 -0.0
GFF2897 +0.4
GFF2983 +0.7
GFF3034 +0.4
GFF3054 -0.5
GFF3395 +0.3
GFF3472 N.D.
GFF3784 +0.3
GFF4130 N.D.
GFF414 +0.2
GFF418 +0.4
GFF4348 -0.0
GFF435 -0.6
GFF436 N.D.
GFF4405 -0.1
GFF4416 +0.3
GFF4496 -0.2
GFF4592 +2.2
GFF4819 -0.0
GFF482 -0.1
GFF4826 -0.0
GFF513 -0.1
GFF5528 +0.4
GFF5614 N.D.
GFF5620 -0.0
GFF5622 +0.3
GFF5663 N.D.
GFF5664 N.D.
GFF5669 N.D.
GFF5848 -0.5
GFF5853 -0.1
GFF5854 +0.0
GFF619 +2.2
GFF620 N.D.
GFF726 -0.1
GFF875 -0.0
GFF882 +0.8
GFF915 -0.1
GFF918 N.D.
GFF931 +0.7
GFF969 -0.2
GFF979 +0.1
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
GFF2109 -0.1
GFF2110 N.D.
GFF3781 -0.1
GFF3825 +0.6
GFF414 +0.2
GFF4497 +0.2
GFF4592 +2.2
GFF4826 -0.0
GFF5620 -0.0
GFF5662 -0.3
GFF875 -0.0
GFF933 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
GFF1723 +1.1
GFF2108 -0.1
GFF3471 +0.2
GFF3554 +0.0
GFF3722 -0.3
GFF3782 -0.0
GFF3813 N.D.
GFF415 -0.1
GFF429 N.D.
GFF437 N.D.
GFF4593 -0.0
GFF4796 +0.0
GFF4825 N.D.
GFF5621 +0.0
GFF5633 -0.3
GFF5665 +0.1
GFF5843 +0.3
GFF678 +0.3
GFF876 +0.2
GFF906 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information