MetaCyc Pathway: glycerol degradation to butanol in Hydrogenophaga sp. GW460-11-11-14-LB1

Add experiment(s):


Reactions and Genes Ying_all64 rep C; time point 3
remove
Glycerol dehydrogenase:
glycerol + NAD+→dihydroxyacetone + NADH + H+ (EC 1.1.1.6)
GFF4376 +0.2
Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
No genes
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
GFF3679 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
GFF1114 -0.5
GFF2202 N.D.
GFF2203 N.D.
GFF2488 N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
GFF2204 N.D.
GFF2205 N.D.
GFF2581 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
GFF1226 N.D.
GFF1661 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
GFF5442 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
GFF2780 +2.1
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
GFF1723 +0.2
GFF2108 +0.0
GFF3471 -0.2
GFF3554 +0.1
GFF3722 +0.0
GFF3782 +0.1
GFF3813 N.D.
GFF415 -0.8
GFF429 N.D.
GFF437 N.D.
GFF4593 -0.4
GFF4796 -0.1
GFF4825 N.D.
GFF5621 +0.1
GFF5633 -0.9
GFF5665 +0.1
GFF5843 +0.4
GFF678 +0.0
GFF876 -0.0
GFF906 -0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
GFF2109 -0.1
GFF376 -0.0
GFF3825 +0.1
GFF414 -0.0
GFF4497 +0.3
GFF4592 +2.0
GFF4826 -0.0
GFF5620 -0.6
GFF5662 +0.1
GFF875 +0.5
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
GFF1003 +0.3
GFF1566 +0.5
GFF2070 N.D.
GFF2072 +0.0
GFF2109 -0.1
GFF2111 -0.5
GFF238 N.D.
GFF2746 +0.1
GFF2842 +0.0
GFF2897 +2.7
GFF2983 +0.8
GFF3034 +0.0
GFF3054 -0.6
GFF3395 -1.9
GFF3472 N.D.
GFF3784 -0.2
GFF4130 N.D.
GFF414 -0.0
GFF418 -0.6
GFF4348 -0.5
GFF435 +0.9
GFF436 N.D.
GFF4405 +0.0
GFF4416 -0.5
GFF4496 -0.0
GFF4592 +2.0
GFF4819 -0.4
GFF482 +1.1
GFF513 -1.1
GFF5528 +1.0
GFF5614 N.D.
GFF5622 -0.6
GFF5663 N.D.
GFF5664 N.D.
GFF5669 N.D.
GFF5848 +0.1
GFF5853 +0.1
GFF5854 +0.5
GFF619 +0.1
GFF620 N.D.
GFF726 +0.8
GFF882 +0.1
GFF915 +1.2
GFF918 N.D.
GFF931 +0.4
GFF969 -0.3
GFF979 -0.2
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information