MetaCyc Pathway: 1-butanol autotrophic biosynthesis (engineered) in Hydrogenophaga sp. GW460-11-11-14-LB1

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Reactions and Genes Ying_all64 rep C; time point 3
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2 H2O[in] + hν[cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + hν[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out] (EC 1.18.1.2)
GFF4298 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H+→D-glyceraldehyde 3-phosphate + NADP+ + phosphate (EC 1.2.1.13)
No genes
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
GFF3679 N.D.
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
GFF2206 -0.5
GFF2777 N.D.
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate (EC 4.1.2.13)
GFF2206 -0.5
GFF2777 N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
GFF2198 -0.0
GFF274 N.D.
Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H2O→D-sedoheptulose 7-phosphate + phosphate (EC 3.1.3.37)
No genes
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
GFF2200 +0.4
GFF2487 N.D.
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)
GFF2200 +0.4
GFF2487 N.D.
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
GFF4212 N.D.
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
GFF2197 +0.7
GFF3096 N.D.
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+ (EC 2.7.1.19)
GFF2199 -0.0
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+ (EC 4.1.1.39)
GFF2193 +0.2
GFF2194 +0.1
GFF5728 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
GFF1226 N.D.
GFF1661 N.D.
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
GFF2204 N.D.
GFF2205 N.D.
GFF2581 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→H2O + phosphoenolpyruvate (EC 4.2.1.11)
GFF5442 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
GFF2780 +2.1
pyruvate + coenzyme A + NAD+→CO2 + acetyl-CoA + NADH (EC 1.2.1.104)
GFF2701 +0.4
GFF4889 -2.8
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
GFF1723 +0.2
GFF2108 +0.0
GFF3471 -0.2
GFF3554 +0.1
GFF3722 +0.0
GFF3782 +0.1
GFF3813 N.D.
GFF415 -0.8
GFF429 N.D.
GFF437 N.D.
GFF4593 -0.4
GFF4796 -0.1
GFF4825 N.D.
GFF5621 +0.1
GFF5633 -0.9
GFF5665 +0.1
GFF5843 +0.4
GFF678 +0.0
GFF876 -0.0
GFF906 -0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
GFF2109 -0.1
GFF376 -0.0
GFF3825 +0.1
GFF414 -0.0
GFF4497 +0.3
GFF4592 +2.0
GFF4826 -0.0
GFF5620 -0.6
GFF5662 +0.1
GFF875 +0.5
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
GFF1003 +0.3
GFF1566 +0.5
GFF2070 N.D.
GFF2072 +0.0
GFF2109 -0.1
GFF2111 -0.5
GFF238 N.D.
GFF2746 +0.1
GFF2842 +0.0
GFF2897 +2.7
GFF2983 +0.8
GFF3034 +0.0
GFF3054 -0.6
GFF3395 -1.9
GFF3472 N.D.
GFF3784 -0.2
GFF4130 N.D.
GFF414 -0.0
GFF418 -0.6
GFF4348 -0.5
GFF435 +0.9
GFF436 N.D.
GFF4405 +0.0
GFF4416 -0.5
GFF4496 -0.0
GFF4592 +2.0
GFF4819 -0.4
GFF482 +1.1
GFF513 -1.1
GFF5528 +1.0
GFF5614 N.D.
GFF5622 -0.6
GFF5663 N.D.
GFF5664 N.D.
GFF5669 N.D.
GFF5848 +0.1
GFF5853 +0.1
GFF5854 +0.5
GFF619 +0.1
GFF620 N.D.
GFF726 +0.8
GFF882 +0.1
GFF915 +1.2
GFF918 N.D.
GFF931 +0.4
GFF969 -0.3
GFF979 -0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
GFF1000 +0.1
GFF1647 -0.0
GFF1714 +0.2
GFF1721 +0.1
GFF1933 -0.3
GFF2069 +0.3
GFF2896 +1.9
GFF3404 +0.3
GFF3468 -0.3
GFF4324 +1.0
GFF4824 -0.2
GFF484 +1.2
GFF5617 -0.1
GFF5631 -0.5
GFF5849 -0.0
GFF5850 -0.1
GFF764 -0.0
GFF865 -0.1
GFF871 N.D.
GFF872 +0.2
GFF877 +0.8
GFF910 +0.5
GFF912 -0.2
GFF919 +0.4
GFF923 +0.4
GFF924 -0.2
GFF930 +0.0
GFF934 -0.3
GFF968 -0.1
GFF975 +0.2
GFF976 +0.3
GFF978 +0.2
GFF988 -0.2
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information