MetaCyc Pathway: hypoglycin biosynthesis in Hydrogenophaga sp. GW460-11-11-14-LB1

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Reactions and Genes Ying_all64 rep C; time point 3
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O
(EC 4.3.1.19)
GFF1379 -3.4
GFF1756 -0.0
GFF1962 +1.2
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate
(spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
GFF1379 -3.4
GFF1756 -0.0
GFF1962 +1.2
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
GFF1379 -3.4
GFF1756 -0.0
GFF1962 +1.2
2-oxobutanoate→5-hydroxy-2-oxopentanoate
No genes
5-hydroxy-2-oxopentanoate→2-oxopent-4-enoate + H2O
No genes
1.-.-.-:
2-oxopent-4-enoate + an oxidized electron carrier→2-oxopenta-3,4-dienoate + a reduced two electron carrier
No genes
2-oxopenta-3,4-dienoate→2-oxohexa-4,5-cyclopropyl-5-enoate
No genes
2-oxohexa-4,5-cyclopropyl-5-enoate + acetyl-CoA + H2O→3-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate + coenzyme A + H+
No genes
2-oxohexa-4,5-cyclopropyl-5-enoate + an aminated amino group donor→L-α-(methylenecyclopropyl)-glycine + a deaminated amino group donor
No genes
3-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate→2-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate
No genes
2-hydroxy-3-carboxy-4,5-cyclopropylhex-5-enoate + an oxidized electron carrier→2-oxo-3-carboxy-4,5-cyclopropylhex-5-enoate + a reduced two electron carrier
No genes
2-oxo-3-carboxy-4,5-cyclopropylhex-5-enoate + H+→β-methylenecyclopropyl pyruvate + CO2
No genes
β-methylenecyclopropyl pyruvate + L-alanine→hypoglycin A + pyruvate
No genes
Gamma-glutamyltransferase:
hypoglycin A + glutathione→hypoglycin B + L-cysteinylglycine
(EC 2.3.2.2)
GFF2135 -0.2
GFF3036 -0.4
GFF3824 -0.4
GFF5191 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information