MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Hydrogenophaga sp. GW460-11-11-14-LB1

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Reactions and Genes Ying_all64 rep C; time point 3
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
GFF1749 -0.1
GFF2040 +1.7
GFF3675 +0.2
GFF3934 +1.6
GFF4304 +0.2
GFF4777 N.D.
GFF4839 -0.0
GFF5171 -0.3
GFF5390 +0.2
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
GFF5136 N.D.
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
GFF3359 N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
GFF3935 N.D.
GFF806 -0.4
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
GFF5586 N.D.
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
GFF3936 N.D.
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
GFF1379 -3.4
GFF1756 -0.0
GFF1962 +1.2
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
GFF1379 -3.4
GFF1756 -0.0
GFF1962 +1.2
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
GFF1379 -3.4
GFF1756 -0.0
GFF1962 +1.2
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
GFF11 N.D.
GFF12 N.D.
GFF1551 +0.0
GFF2148 -0.3
GFF2981 -0.4
GFF3744 N.D.
GFF3745 N.D.
GFF3965 +0.1
GFF4276 N.D.
GFF4277 +3.8
GFF445 -0.2
GFF5606 -0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
GFF4278 N.D.
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
GFF2073 -0.1
GFF2098 -0.2
GFF4111 N.D.
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
GFF1835 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information