MetaCyc Pathway: L-isoleucine degradation I in Acinetobacter baumannii LAC-4

Add experiment(s):


Reactions and Genes Right Thigh 3
remove
Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)
RR41_RS02965 +0.3
2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD+→(2S)-2-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
(E)-2-methylcrotonoyl-CoA + H2O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)
RR41_RS03215 +0.3
RR41_RS04720 -0.4
RR41_RS08640 +1.0
RR41_RS11015 +0.1
RR41_RS16105 -0.3
RR41_RS17450 -1.0
RR41_RS18325 -0.0
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+→2-methylacetoacetyl-CoA + NADH + H+ (EC 1.1.1.178)
RR41_RS03215 +0.3
RR41_RS06885 +0.2
RR41_RS10510 -0.2
RR41_RS17450 -1.0
Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)
RR41_RS03220 -0.6
RR41_RS03265 +0.2
RR41_RS07405 -0.5
RR41_RS10890 -0.2
RR41_RS11035 +1.5
RR41_RS12900 +0.5
RR41_RS13605 N.D.
RR41_RS13800 +0.4
RR41_RS17455 -0.8

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information