MetaCyc Pathway: gluconeogenesis I in Escherichia coli ECRC101

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Reactions and Genes Casamino-acids
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Malate dehydrogenase (oxaloacetate-decarboxylating):
(S)-malate + NAD+→pyruvate + CO2 + NADH (EC 1.1.1.38)
MCAODC_16580 (maeA) -0.1
Malate dehydrogenase:
(S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
MCAODC_08315 (mdh) -3.3
MCAODC_16580 (maeA) -0.1
MCAODC_25485 (hcxB) -0.1
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP+→pyruvate + CO2 + NADPH (EC 1.1.1.40)
MCAODC_12535 (maeB) +0.2
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→phosphoenolpyruvate + ADP + CO2 (EC 4.1.1.49)
MCAODC_07575 (pckA) +0.0
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
MCAODC_18265 (ppsA) -0.2
Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H2O→2-phospho-D-glycerate (EC 4.2.1.11)
MCAODC_10800 (eno) N.D.
2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.12)
MCAODC_01670 -0.1
MCAODC_06245 (gpmM) -0.1
MCAODC_25955 (gpmA) -0.3
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
MCAODC_09910 (pgk) N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADH + H+→D-glyceraldehyde 3-phosphate + NAD+ + phosphate (EC 1.2.1.12)
MCAODC_16240 (gap) +0.0
MCAODC_18680 (gapA) N.D.
Fructose-bisphosphate aldolase (in reverse):
D-glyceraldehyde 3-phosphate + glycerone phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
MCAODC_03240 (fba) +0.2
MCAODC_07010 (fba) +0.2
MCAODC_09915 (fbaA) N.D.
MCAODC_21055 (fbaB) +0.1
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
MCAODC_02450 (fbp) -4.6
MCAODC_04280 (glpX) +0.1
MCAODC_09865 (glpX) -0.0
MCAODC_25900 (ybhA) -0.1
MCAODC_27850 (phoA) +0.0
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
MCAODC_04310 (tpiA) N.D.
Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)
MCAODC_03675 (pgi) -0.3
MCAODC_10445 (kduI) -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information