MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Escherichia coli ECRC101

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Reactions and Genes L-Arabinose
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
MCAODC_01210 (ilvN) -0.0
MCAODC_01215 (ilvI) +0.1
MCAODC_05155 -1.0
MCAODC_05160 (ilvG) -1.0
MCAODC_05660 -1.1
MCAODC_05665 (ilvN) +0.3
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
MCAODC_05130 (ilvC) -3.2
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
MCAODC_05145 (ilvD) -2.5
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
MCAODC_02275 (ahr) +0.3
MCAODC_06370 (yiaY) +0.0
MCAODC_08235 (acuI) +0.3
MCAODC_16575 (adhP) -0.6
MCAODC_21655 (adhE) +0.2
MCAODC_27970 (frmA) -0.3
MCAODC_28155 (yahK) +0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information