MetaCyc Pathway: TCA cycle I (prokaryotic) in Escherichia coli ECRC101

Reactions and Genes	Casamino-acids
2-oxoglutarate + coenzyme A + NAD+→succinyl-CoA + CO2 + NADH (EC 1.2.1.105)
MCAODC_01005 (lpdA)	N.D.
Succinate--CoA ligase (ADP-forming) (in reverse): succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)
MCAODC_26130 (sucD)	-5.9
MCAODC_26135 (sucC)	-4.0
Succinate dehydrogenase (ubiquinone): succinate[in] + an electron-transfer quinone→fumarate[in] + an electron-transfer quinol (EC 1.3.5.1)
No genes
Fumarate hydratase (in reverse): fumarate + H2O→(S)-malate (EC 4.2.1.2)
MCAODC_03035 (fumB)	+0.2
MCAODC_09870 (fumE)	+0.1
MCAODC_17760 (fumC)	+0.0
MCAODC_17765 (fumB)	-2.0
MCAODC_18115 (fumD)	-0.2
MCAODC_26115 (ttdA)	+0.1
Malate dehydrogenase: (S)-malate + NAD+→oxaloacetate + NADH + H+ (EC 1.1.1.37; 1.1.1.38)
MCAODC_08315 (mdh)	-3.6
MCAODC_16580 (maeA)	+0.2
MCAODC_25485 (hcxB)	-0.3
(S)-malate[in] + an electron-transfer quinone→oxaloacetate[in] + an electron-transfer quinol (EC 1.1.5.4)
MCAODC_14275 (mqo)	+0.1
Citrate (Si)-synthase: oxaloacetate + acetyl-CoA + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
MCAODC_26180 (gltA)	-2.1
Aconitate hydratase: citrate→cis-aconitate + H2O (EC 4.2.1.3)
MCAODC_00995 (acnB)	-3.0
MCAODC_15690 (acnA)	+0.3
MCAODC_25875 (ybhJ)	-0.0
Aconitate hydratase: cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
MCAODC_00995 (acnB)	-3.0
MCAODC_15690 (acnA)	+0.3
MCAODC_25875 (ybhJ)	-0.0
Isocitrate dehydrogenase (NADP(+)): D-threo-isocitrate + NADP+→2-oxoglutarate + CO2 + NADPH (EC 1.1.1.42)
MCAODC_22325 (icd)	N.D.

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information