MetaCyc Pathway: mixed acid fermentation in Escherichia coli ECRC101

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Reactions and Genes Sodium-DL-Lactate
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Phosphoenolpyruvate carboxylase (in reverse):
phosphoenolpyruvate + hydrogen carbonate→oxaloacetate + phosphate (EC 4.1.1.31)
MCAODC_04095 (ppc) -5.6
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
MCAODC_18120 (pykF) N.D.
MCAODC_19085 (pyk) -0.2
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
MCAODC_14700 (dld) -0.5
MCAODC_16140 (ldhA) +0.1
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
MCAODC_08315 (mdh) -3.5
MCAODC_16580 (maeA) +0.1
MCAODC_25485 (hcxB) -0.2
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
MCAODC_04115 (pflC) -0.3
MCAODC_04120 (pflD) -0.0
MCAODC_08930 (tdcE) -0.1
MCAODC_11875 (grcA) +0.0
MCAODC_24900 (pflB) -0.1
MCAODC_24905 (pflA) +0.8
MCAODC_25350 (ybiY) -0.3
MCAODC_25355 +0.1
MCAODC_25360 (pflD) -0.1
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
MCAODC_12575 (eutE) -0.1
MCAODC_21655 (adhE) -0.1
MCAODC_27995 (mhpF) -0.3
Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H2O→citrate + coenzyme A + H+ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)
MCAODC_26180 (gltA) -4.1
formate + H+→CO2 + H2
MCAODC_03280 (fdhF) +0.0
MCAODC_11100 (hycB) -0.4
MCAODC_11105 (hycC) -0.0
MCAODC_11110 (hycD) +0.6
MCAODC_12420 (hyfC) +0.0
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
MCAODC_03035 (fumB) -0.0
MCAODC_09870 (fumE) -0.3
MCAODC_17760 (fumC) -1.6
MCAODC_17765 (fumB) -0.3
MCAODC_18115 (fumD) -0.2
MCAODC_26115 (ttdA) -0.1
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
MCAODC_12560 (pta) +0.0
MCAODC_13830 (pta) N.D.
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
MCAODC_08925 (tdcD) -0.0
MCAODC_12545 (eutP) +0.1
MCAODC_12550 (eutQ) +0.1
MCAODC_13835 (ackA) N.D.
MCAODC_19055 (purT) +0.2
Aconitate hydratase:
citrate→cis-aconitate + H2O (EC 4.2.1.3)
MCAODC_00995 (acnB) -3.1
MCAODC_15690 (acnA) +0.3
MCAODC_25875 (ybhJ) +0.1
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
MCAODC_02275 (ahr) -0.2
MCAODC_06370 (yiaY) -0.0
MCAODC_08235 (acuI) +0.1
MCAODC_16575 (adhP) -0.3
MCAODC_21655 (adhE) -0.1
MCAODC_27970 (frmA) -0.2
MCAODC_28155 (yahK) +0.2
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
MCAODC_02855 (frdA) +0.0
MCAODC_02860 (frdB) +0.0
MCAODC_02865 +0.2
MCAODC_02870 (frdD) -0.7
Aconitate hydratase:
cis-aconitate + H2O→D-threo-isocitrate (EC 4.2.1.3)
MCAODC_00995 (acnB) -3.1
MCAODC_15690 (acnA) +0.3
MCAODC_25875 (ybhJ) +0.1
Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP+→CO2 + 2-oxoglutarate + NADPH (EC 1.1.1.42)
MCAODC_22325 (icd) N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information