MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Escherichia coli ECRC101

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Reactions and Genes L-Arabinose
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
MCAODC_05140 (ilvA) -3.4
MCAODC_08915 (tdcB) +0.5
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
MCAODC_05140 (ilvA) -3.4
MCAODC_08915 (tdcB) +0.5
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
MCAODC_02380 (ridA) -0.4
MCAODC_05140 (ilvA) -3.4
MCAODC_08915 (tdcB) +0.5
MCAODC_08935 (tdcF) +0.2
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
MCAODC_01210 (ilvN) -0.1
MCAODC_01215 (ilvI) -0.0
MCAODC_05155 -0.8
MCAODC_05160 (ilvG) -1.1
MCAODC_05660 -1.5
MCAODC_05665 (ilvN) -1.0
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
MCAODC_05130 (ilvC) -3.6
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
MCAODC_05145 (ilvD) -2.9
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
MCAODC_05150 (ilvE) -4.6

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information