MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Pseudomonas simiae WCS417

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Reactions and Genes L-Valine (C)
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
PS417_12665 -3.3
PS417_12925 +0.8
PS417_27010 -2.6
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
PS417_11735 +0.6
PS417_12665 -3.3
PS417_12925 +0.8
PS417_17160 +1.0
PS417_27010 -2.6
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
PS417_11735 +0.6
PS417_12665 -3.3
PS417_12925 +0.8
PS417_17160 +1.0
PS417_27010 -2.6
PS417_27835 -7.0
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
PS417_07295 +0.1
PS417_08225 -1.9
PS417_10695 -0.1
PS417_13315 +0.1
PS417_23805 -5.9
PS417_23810 -5.7
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
PS417_23800 -6.1
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
PS417_11000 -0.2
PS417_26890 -4.4
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
PS417_17565 -6.8

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information