MetaCyc Pathway: (5Z)-dodecenoate biosynthesis I in Pseudomonas sp. RS175

Add experiment(s):


Reactions and Genes D-(+)-Galactosamine (C) 10 mM
remove
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydecanoyl-[acp]→a (2E)-dec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
PFR28_00407 N.D.
PFR28_03080 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dec-2-enoyl-[acp]→a (3Z)-dec-3-enoyl-[acp] (EC 5.3.3.14)
PFR28_03080 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxododec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
PFR28_01955 -0.4
PFR28_02356 +0.1
PFR28_03008 N.D.
PFR28_03081 N.D.
PFR28_05021 -0.3
PFR28_05024 +0.1
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxododec-5-enoyl-[acp] + NADPH + H+→a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp] + NADP+ (EC 1.1.1.100)
PFR28_01793 -0.1
PFR28_01903 +0.1
PFR28_01921 -0.0
PFR28_02161 -0.1
PFR28_02545 -0.2
PFR28_02939 +0.0
PFR28_02995 -1.5
PFR28_03010 N.D.
PFR28_03341 +0.1
PFR28_04605 -0.0
PFR28_04858 -0.1
PFR28_05022 +0.3
PFR28_05238 +0.1
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxydodec-5-enoyl-[acp]→a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + H2O (EC 4.2.1.59)
PFR28_00407 N.D.
PFR28_03080 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-dodeca-3,5-dienoyl-[acp] + NADH + H+→a (5Z)-dodec-5-enoyl-[acp] + NAD+ (EC 1.3.1.9)
PFR28_01545 N.D.
PFR28_02495 +0.1

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information