MetaCyc Pathway: (S)-reticuline biosynthesis I in Pseudomonas fluorescens GW456-L13

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Reactions and Genes L-Phenylalanine (C)
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Monophenol monooxygenase:
2 L-tyrosine + dioxygen→2 L-dopa (EC 1.14.18.1)
No genes
Aspartate transaminase:
L-tyrosine + 2-oxoglutarate→3-(4-hydroxyphenyl)pyruvate + L-glutamate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
PfGW456L13_2059 +0.2
PfGW456L13_2142 -0.7
PfGW456L13_2177 -0.4
PfGW456L13_2252 -0.1
PfGW456L13_2943 +0.0
PfGW456L13_2947 +0.3
PfGW456L13_3163 +0.0
PfGW456L13_3206 +0.2
PfGW456L13_3261 +0.1
PfGW456L13_3292 +0.2
PfGW456L13_3363 +0.2
PfGW456L13_3960 -0.0
PfGW456L13_4175 +0.1
PfGW456L13_4396 -1.7
PfGW456L13_4856 -0.1
PfGW456L13_4919 +0.1
PfGW456L13_4930 +0.3
PfGW456L13_4999 -1.7
PfGW456L13_733 -0.1
PfGW456L13_807 -0.1
Tyrosine decarboxylase:
L-tyrosine + H+→tyramine + CO2 (EC 4.1.1.25; 4.1.1.28)
No genes
4-hydroxyphenylpyruvate decarboxylase:
3-(4-hydroxyphenyl)pyruvate + H+→(4-hydroxyphenyl)acetaldehyde + CO2 (EC 4.1.1.80)
No genes
Tyrosine 3-monooxygenase:
tyramine + (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + dioxygen→dopamine + 4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin (EC 1.14.16.2)
No genes
Aromatic-L-amino-acid decarboxylase:
L-dopa + H+→dopamine + CO2 (EC 4.1.1.28)
No genes
(S)-norcoclaurine synthase:
dopamine + (4-hydroxyphenyl)acetaldehyde→(S)-norcoclaurine + H2O (EC 4.2.1.78)
No genes
(RS)-norcoclaurine 6-O-methyltransferase:
(S)-norcoclaurine + S-adenosyl-L-methionine→(S)-coclaurine + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.128)
No genes
(S)-coclaurine-N-methyltransferase:
(S)-coclaurine + S-adenosyl-L-methionine→(S)-N-methylcoclaurine + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.140)
No genes
(S)-N-methylcoclaurine + dioxygen + a reduced [NADPH-hemoprotein reductase]→(S)-3'-hydroxy-N-methylcoclaurine + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.102)
No genes
3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase:
(S)-3'-hydroxy-N-methylcoclaurine + S-adenosyl-L-methionine→(S)-reticuline + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.116)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information