MetaCyc Pathway: formaldehyde assimilation III (dihydroxyacetone cycle) in Escherichia coli BW25113

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Reactions and Genes 2-Furfuraldehyde 0.25 vol%
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Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
b2465 (tktB) -0.0
b2935 (tktA) -1.4
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
b2914 (rpiA) N.D.
b4090 (rpiB) -0.0
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
b3386 (rpe) -0.5
b4301 (sgcE) -0.0
Formaldehyde transketolase:
D-xylulose 5-phosphate + formaldehyde→D-glyceraldehyde 3-phosphate + dihydroxyacetone (EC 2.2.1.3)
No genes
Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
No genes
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
b2097 (b2097) -0.0
b2925 (fbaA) N.D.
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
b3919 (tpiA) N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
b0383 (phoA) -0.2
b0766 (ybhA) -0.0
b2930 (yggF) +0.1
b3925 (glpX) +0.1
b4232 (fbp) -0.1
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA) N.D.
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)
b2465 (tktB) -0.0
b2935 (tktA) -1.4
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk) N.D.
Transaldolase (in reverse):
β-D-fructofuranose 6-phosphate + D-erythrose 4-phosphate→D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate (EC 2.2.1.2)
b0008 (talB) +1.5
b0825 (mipB) -0.4
b2464 (talA) -0.3
b3946 (fsaB) -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information