MetaCyc Pathway: Bifidobacterium shunt in Escherichia coli BW25113

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Reactions and Genes 2-Furfuraldehyde 0.25 vol%
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Hexokinase:
D-glucopyranose + ATP→D-glucopyranose 6-phosphate + ADP + H+ (EC 2.7.1.1; 2.7.1.2)
b0394 (yajF) -0.1
b2388 (glk) +0.1
Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
b2843 (kduI) +0.1
b4025 (pgi) -1.7
Fructose-6-phosphate phosphoketolase:
β-D-fructofuranose 6-phosphate + phosphate→acetyl phosphate + D-erythrose 4-phosphate + H2O (EC 4.1.2.22)
No genes
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
b1849 (purT) -0.4
b2296 (ackA) N.D.
b2460 (eutQ) -0.1
b2461 (eutP) -0.1
b3115 (tdcD) -0.1
Transaldolase (in reverse):
D-erythrose 4-phosphate + β-D-fructofuranose 6-phosphate→D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate (EC 2.2.1.2)
b0008 (talB) +1.5
b0825 (mipB) -0.4
b2464 (talA) -0.3
b3946 (fsaB) -0.0
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
b2465 (tktB) -0.0
b2935 (tktA) -1.4
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
b2914 (rpiA) N.D.
b4090 (rpiB) -0.0
Ribulose-phosphate 3-epimerase:
D-ribulose 5-phosphate→D-xylulose 5-phosphate (EC 5.1.3.1)
b3386 (rpe) -0.5
b4301 (sgcE) -0.0
Phosphoketolase:
D-xylulose 5-phosphate + phosphate→acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O (EC 4.1.2.9)
No genes
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
b1779 (gapA) N.D.
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
b2926 (pgk) N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
b0755 (gpmA) +0.7
b3612 (gpmI) -0.0
b4395 (ytjC) +0.3
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
b2779 (eno) N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
b1676 (pykF) +1.5
b1854 (pykA) +0.5
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
b0306 (ykgE) +0.1
b0307 (ykgF) +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information