MetaCyc Pathway: oleate biosynthesis IV (anaerobic) in Agrobacterium fabrum C58

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Reactions and Genes Melibiose (C)
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3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxydodecanoyl-[acp]→a (2E)-dodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Trans-2-decenoyl-[acyl-carrier-protein] isomerase:
a (2E)-dodec-2-enoyl-[acp]→a (3Z)-dodec-3-enoyl-[acp] (EC 5.3.3.14)
No genes
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (3Z)-dodec-3-enoyl-[acp] + a malonyl-[acp] + H+→a (5Z)-3-oxo-tetradec-5-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Atu0150 N.D.
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (5Z)-3-oxo-tetradec-5-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
Atu0705 -2.1
Atu0990 +0.0
Atu1095 N.D.
Atu1255 -0.2
Atu1338 -0.1
Atu2042 -0.1
Atu3051 -0.0
Atu3074 -0.2
Atu3333 -0.0
Atu3531 +0.0
Atu3813 -0.0
Atu4220 -0.0
Atu4325 -0.0
Atu4595 +0.2
Atu4821 -0.1
Atu4877 +0.2
Atu5122 +0.0
Atu5214 N.D.
Atu5250 N.D.
Atu5438 -0.0
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,5Z)-3-hydroxy-tetradec-5-enoyl-[acp][cytosol]→a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,5Z)-tetradeca-2,5-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (5Z)-tetradec-5-enoyl-[acp][cytosol] + NAD+[cytosol] (EC 1.3.1.9)
Atu0149 +0.1
Atu0757 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (5Z)-tetradec-5-enoyl-[acp] + a malonyl-[acp] + H+→a (7Z)-3-oxo-hexadec-7-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Atu0150 N.D.
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (7Z)-3-oxo-hexadec-7-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
Atu0705 -2.1
Atu0990 +0.0
Atu1095 N.D.
Atu1255 -0.2
Atu1338 -0.1
Atu2042 -0.1
Atu3051 -0.0
Atu3074 -0.2
Atu3333 -0.0
Atu3531 +0.0
Atu3813 -0.0
Atu4220 -0.0
Atu4325 -0.0
Atu4595 +0.2
Atu4821 -0.1
Atu4877 +0.2
Atu5122 +0.0
Atu5214 N.D.
Atu5250 N.D.
Atu5438 -0.0
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,7Z)-3-hydroxy-hexadec-7-enoyl-[acp][cytosol]→a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,7Z)-hexadeca-2,7-dienoyl-[acp][cytosol] + NADH[cytosol] + H+[cytosol]→a (7Z)-hexadec-7-enoyl-[acp][cytosol] + NAD+[cytosol] (EC 1.3.1.9)
Atu0149 +0.1
Atu0757 N.D.
Beta-ketoacyl-acyl-carrier-protein synthase II:
a (7Z)-hexadec-7-enoyl-[acp] + a malonyl-[acp] + H+→a (9Z)-3-oxo-octadec-9-enoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179; 2.3.1.41)
Atu0150 N.D.
Atu1097 N.D.
Atu1596 N.D.
Atu1597 N.D.
Atu4216 -0.0
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a (9Z)-3-oxo-octadec-9-enoyl-[acp][cytosol] + NADPH[cytosol] + H+[cytosol]→a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol] + NADP+[cytosol] (EC 1.1.1.100)
Atu0705 -2.1
Atu0990 +0.0
Atu1095 N.D.
Atu1255 -0.2
Atu1338 -0.1
Atu2042 -0.1
Atu3051 -0.0
Atu3074 -0.2
Atu3333 -0.0
Atu3531 +0.0
Atu3813 -0.0
Atu4220 -0.0
Atu4325 -0.0
Atu4595 +0.2
Atu4821 -0.1
Atu4877 +0.2
Atu5122 +0.0
Atu5214 N.D.
Atu5250 N.D.
Atu5438 -0.0
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R,9Z)-3-hydroxy-octadec-9-enoyl-[acp][cytosol]→a (2E,9Z)-octadeca-2,9-dienoyl-[acp][cytosol] + H2O[cytosol] (EC 4.2.1.59)
Atu0151 N.D.
Atu1383 N.D.
Atu1599 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E,9Z)-octadeca-2,9-dienoyl-[acp] + NADH + H+→an oleoyl-[acp] + NAD+ (EC 1.3.1.9)
Atu0149 +0.1
Atu0757 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information