MetaCyc Pathway: superpathway of L-alanine biosynthesis in Escherichia coli BW25113

Reactions and Genes	Nickel (II) chloride 1 mM
Alanine transaminase (in reverse): pyruvate + L-glutamate→L-alanine + 2-oxoglutarate (EC 2.6.1.2)
b2290 (yfbQ)	+0.1
b2379 (yfdZ)	-0.1
Branched-chain-amino-acid transaminase (in reverse): 3-methyl-2-oxobutanoate + L-glutamate→L-valine + 2-oxoglutarate (EC 2.6.1.42)
b3770 (ilvE)	-0.0
Cysteine desulfurase: L-cysteine + an unsulfurated [sulfur carrier]→L-alanine + a sulfurated [sulfur carrier] (EC 2.8.1.7)
b1680 (sufS)	+0.1
b2810 (csdA)	+0.0
b2811 (ygdK)	+0.2
b2530 (iscS)	N.D.
Valine--pyruvate transaminase: L-valine + pyruvate→3-methyl-2-oxobutanoate + L-alanine (EC 2.6.1.66)
b2290 (yfbQ)	+0.1
b3572 (avtA)	+1.5

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information