MetaCyc Pathway: colanic acid building blocks biosynthesis in Escherichia coli BW25113

Reactions and Genes	Chloride 750 mM
Glucose-6-phosphate isomerase: α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)
b2843 (kduI)	+0.1
b4025 (pgi)	-0.2
Phosphoglucomutase (in reverse): D-glucopyranose 6-phosphate→α-D-glucopyranose 1-phosphate (EC 5.4.2.2)
b0688 (pgm)	+0.0
b3176 (glmM)	N.D.
UTP-monosaccharide-1-phosphate uridylyltransferase: α-D-glucopyranose 1-phosphate + H+ + UTP→UDP-α-D-glucose + diphosphate (EC 2.7.7.64; 2.7.7.9)
b1236 (galU)	+0.4
b2042 (galF)	-0.1
Mannose-6-phosphate isomerase (in reverse): β-D-fructofuranose 6-phosphate→D-mannopyranose 6-phosphate (EC 5.3.1.8)
b1613 (manA)	-1.0
b2049 (cpsB)	-0.4
Phosphomannomutase (in reverse): D-mannopyranose 6-phosphate→α-D-mannose 1-phosphate (EC 5.4.2.8)
b2048 (cpsG)	-0.9
UDP-glucose 4-epimerase: UDP-α-D-glucose→UDP-α-D-galactose (EC 5.1.3.2)
b0759 (galE)	-1.8
UDP-glucose 6-dehydrogenase: UDP-α-D-glucose + 2 NAD+ + H2O→UDP-α-D-glucuronate + 2 NADH + 3 H+ (EC 1.1.1.22)
b2028 (ugd)	-2.6
b3787 (rffD)	-1.2
Mannose-1-phosphate guanylyltransferase: α-D-mannose 1-phosphate + GTP + H+→GDP-α-D-mannose + diphosphate (EC 2.7.7.13)
b2049 (cpsB)	-0.4
GDP-mannose 4,6-dehydratase: GDP-α-D-mannose→GDP-4-dehydro-α-D-rhamnose + H2O (EC 4.2.1.47)
b2053 (gmd)	-0.9
GDP-L-fucose synthase: GDP-4-dehydro-α-D-rhamnose→GDP-4-dehydro-6-deoxy-β-L-galactose (EC 1.1.1.271)
b2052 (fcl)	-1.0
GDP-L-fucose synthase (in reverse): GDP-4-dehydro-6-deoxy-β-L-galactose + NADPH + H+→GDP-β-L-fucose + NADP+ (EC 1.1.1.271)
b2052 (fcl)	-1.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information