MetaCyc Pathway: benzoate biosynthesis I (CoA-dependent, β-oxidative) in Pseudomonas simiae WCS417

Add experiment(s):


Reactions and Genes LB_noSalt with Chloride 1100 mM
remove
Phenylalanine ammonia-lyase:
L-phenylalanine→trans-cinnamate + ammonium (EC 4.3.1.24; 4.3.1.25; 5.4.3.10)
No genes
trans-cinnamate + ATP + coenzyme A→(E)-cinnamoyl-CoA + AMP + diphosphate
No genes
Enoyl-CoA hydratase (in reverse):
(E)-cinnamoyl-CoA + H2O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)
PS417_07030 +0.2
PS417_07575 -0.6
PS417_08535 -0.4
PS417_10680 -0.3
PS417_11445 -2.2
PS417_12870 -0.2
PS417_13835 -0.2
PS417_13845 N.D.
PS417_21195 +0.0
PS417_21215 -0.0
PS417_22625 -0.1
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD+→3-oxo-3-phenylpropanoyl-CoA + NADH + H+ (EC 1.1.1.35)
PS417_07575 -0.6
PS417_21215 -0.0
PS417_26285 -0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)
PS417_06710 +0.0
PS417_07580 N.D.
PS417_10515 -0.0
PS417_13855 -0.4
PS417_15175 N.D.
PS417_19210 -0.1
PS417_21220 -0.2
PS417_03145 +0.0
benzoyl-CoA + H2O→benzoate + coenzyme A + H+
No genes
benzoyl-CoA + benzyl alcohol→benzyl benzoate + coenzyme A (EC 2.3.1.196)
No genes
benzyl benzoate + H2O→benzoate + benzyl alcohol + H+
No genes
Benzoate--CoA ligase:
benzoate + ATP + coenzyme A→benzoyl-CoA + AMP + diphosphate (EC 6.2.1.25)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information