MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Pseudomonas simiae WCS417

Add experiment(s):


Reactions and Genes LB_noSalt with Chloride 1100 mM
remove
Glutamate dehydrogenase (NAD(P)(+)) (in reverse):
2-oxoglutarate + ammonium + NAD(P)H + H+→L-glutamate + NAD(P)+ + H2O (EC 1.4.1.3)
No genes
Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)
PS417_05415 N.D.
Glutamate synthase (NADH) (in reverse):
2-oxoglutarate + L-glutamine + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
PS417_17325 +0.0
PS417_02005 -0.3
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
PS417_05250 +0.1
PS417_05415 N.D.
PS417_06300 N.D.
PS417_06580 +0.0
PS417_06685 +0.1
PS417_08425 +0.1
PS417_11435 +0.2
PS417_15505 +0.2
PS417_16695 -0.2
PS417_17275 +0.2
PS417_17325 +0.0
PS417_17690 -0.1
PS417_17915 -0.2
PS417_18410 +0.1
PS417_19230 -1.3
PS417_19985 N.D.
PS417_02000 -0.1
PS417_02005 -0.3
PS417_21105 +0.3
PS417_21210 +0.1
PS417_21855 +0.2
PS417_22155 -0.5
PS417_22930 N.D.
PS417_23070 N.D.
PS417_24040 N.D.
PS417_24045 N.D.
PS417_25280 -0.2
PS417_25720 +0.3
PS417_25725 -0.0
PS417_28355 +0.2
PS417_04235 N.D.
PS417_04240 N.D.
PS417_04245 N.D.
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
PS417_07365 -0.1
PS417_10580 -0.0
PS417_11785 +0.3
PS417_01655 N.D.
PS417_27135 +0.6
PS417_27140 +0.2
Glutamate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + ammonium + NADPH + H+→L-glutamate + NADP+ + H2O (EC 1.4.1.4)
PS417_02005 -0.3
PS417_24260 -0.1
Glutamate synthase (NADPH) (in reverse):
2-oxoglutarate + ammonia + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
PS417_06580 +0.0
PS417_06685 +0.1
PS417_08425 +0.1
PS417_16695 -0.2
PS417_17275 +0.2
PS417_17325 +0.0
PS417_17915 -0.2
PS417_19985 N.D.
PS417_02000 -0.1
PS417_02005 -0.3
PS417_22155 -0.5
PS417_22930 N.D.
PS417_25280 -0.2
PS417_28355 +0.2

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information