MetaCyc Pathway: 3-phenylpropanoate degradation in Pseudomonas simiae WCS417

Reactions and Genes	LB_noSalt with Chloride 1100 mM
3-phenylpropanoate + ATP + coenzyme A→3-phenylpropanoyl-CoA + AMP + diphosphate
No genes
3-phenylpropanoyl-CoA + an oxidized electron carrier→(E)-cinnamoyl-CoA + a reduced two electron carrier
No genes
Enoyl-CoA hydratase (in reverse): (E)-cinnamoyl-CoA + H2O→(S)-3-hydroxy-3-phenylpropanoyl-CoA (EC 4.2.1.17)
PS417_07030	+0.2
PS417_07575	-0.6
PS417_08535	-0.4
PS417_10680	-0.3
PS417_11445	-2.2
PS417_12870	-0.2
PS417_13835	-0.2
PS417_13845	N.D.
PS417_21195	+0.0
PS417_21215	-0.0
PS417_22625	-0.1
3-hydroxyacyl-CoA dehydrogenase: (S)-3-hydroxy-3-phenylpropanoyl-CoA + NAD+→3-oxo-3-phenylpropanoyl-CoA + NADH + H+ (EC 1.1.1.35)
PS417_07575	-0.6
PS417_21215	-0.0
PS417_26285	-0.1
Acetyl-CoA C-acyltransferase (in reverse): 3-oxo-3-phenylpropanoyl-CoA + coenzyme A→benzoyl-CoA + acetyl-CoA (EC 2.3.1.16)
PS417_06710	+0.0
PS417_07580	N.D.
PS417_10515	-0.0
PS417_13855	-0.4
PS417_15175	N.D.
PS417_19210	-0.1
PS417_21220	-0.2
PS417_03145	+0.0
Benzoyl-CoA 3-monooxygenase: benzoyl-CoA + NADPH + dioxygen + H+→3-hydroxybenzoyl-CoA + NADP+ + H2O (EC 1.14.13.58)
No genes
3-hydroxybenzoyl-CoA + a reduced two electron carrier + dioxygen→gentisate + an oxidized electron carrier + coenzyme A + H+
No genes
Gentisate 1,2-dioxygenase: gentisate + dioxygen→3-maleylpyruvate + H+ (EC 1.13.11.4)
No genes
Maleylpyruvate isomerase: 3-maleylpyruvate→3-fumarylpyruvate (EC 5.2.1.4)
No genes
3-fumarylpyruvate + H2O→fumarate + pyruvate + H+ (EC 3.7.1.20)
No genes

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information