MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Pseudomonas simiae WCS417

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Reactions and Genes LB_noSalt with Chloride 1100 mM
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Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
PS417_04135 -0.1
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
PS417_13840 +0.2
PS417_13850 -0.3
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
PS417_02170 -0.0
PS417_24590 -0.4
PS417_25960 -0.1
PS417_25965 -0.2
PS417_25975 -0.3
PS417_04135 -0.1
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PS417_07030 +0.2
PS417_07575 -0.6
PS417_08535 -0.3
PS417_10680 -0.6
PS417_11445 -2.2
PS417_12870 -0.3
PS417_13835 -0.2
PS417_13845 N.D.
PS417_21195 +0.1
PS417_21215 +0.1
PS417_22625 -0.0
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PS417_07030 +0.2
PS417_07575 -0.6
PS417_08535 -0.3
PS417_10680 -0.6
PS417_11445 -2.2
PS417_12870 -0.3
PS417_13835 -0.2
PS417_13845 N.D.
PS417_21195 +0.1
PS417_21215 +0.1
PS417_22625 -0.0
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
PS417_07030 +0.2
PS417_07575 -0.6
PS417_08535 -0.3
PS417_10680 -0.6
PS417_11445 -2.2
PS417_12870 -0.3
PS417_13835 -0.2
PS417_13845 N.D.
PS417_21195 +0.1
PS417_21215 +0.1
PS417_22625 -0.0
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
PS417_07575 -0.6
PS417_21215 +0.1
PS417_26285 +0.0
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
No genes
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
PS417_07575 -0.6
PS417_21215 +0.1
PS417_26285 +0.0
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PS417_06710 -0.4
PS417_07580 N.D.
PS417_10515 -0.2
PS417_13855 +0.3
PS417_15175 N.D.
PS417_19210 +0.1
PS417_21220 -0.3
PS417_03145 -0.4
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PS417_06710 -0.4
PS417_07580 N.D.
PS417_10515 -0.2
PS417_13855 +0.3
PS417_15175 N.D.
PS417_19210 +0.1
PS417_21220 -0.3
PS417_03145 -0.4
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
PS417_06710 -0.4
PS417_07580 N.D.
PS417_10515 -0.2
PS417_13855 +0.3
PS417_15175 N.D.
PS417_19210 +0.1
PS417_21220 -0.3
PS417_03145 -0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information