MetaCyc Pathway: (8E,10E)-dodeca-8,10-dienol biosynthesis in Pseudomonas simiae WCS417

Add experiment(s):


Reactions and Genes LB_noSalt with Chloride 1100 mM
remove
palmitoyl-CoA[in] + an oxidized electron-transfer flavoprotein[in] + H+[in]→(2E)-hexadec-2-enoyl-CoA[in] + a reduced electron-transfer flavoprotein[in] (EC 1.3.8.9)
No genes
(8E,10E)-dodeca-8,10-dienoate + 2 NADPH + 3 H+→(8E,10E)-dodeca-8,10-dienol + 2 NADP+ + H2O
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-hexadec-2-enoyl-CoA + H2O→(S)-3-hydroxyhexadecanoyl-CoA (EC 4.2.1.17)
PS417_07030 +0.2
PS417_07575 -0.6
PS417_08535 -0.3
PS417_10680 -0.6
PS417_11445 -2.2
PS417_12870 -0.3
PS417_13835 -0.2
PS417_13845 N.D.
PS417_21195 +0.1
PS417_21215 +0.1
PS417_22625 -0.0
Long-chain-3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxyhexadecanoyl-CoA + NAD+→3-oxopalmitoyl-CoA + NADH + H+ (EC 1.1.1.211)
PS417_07575 -0.6
PS417_21215 +0.1
Acetyl-CoA C-myristoyltransferase (in reverse):
3-oxopalmitoyl-CoA + coenzyme A→myristoyl-CoA + acetyl-CoA (EC 2.3.1.155)
PS417_13855 +0.3
PS417_19210 +0.1
myristoyl-CoA + an oxidized electron-transfer flavoprotein + H+→(2E)-tetradec-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Enoyl-CoA hydratase (in reverse):
(2E)-tetradec-2-enoyl-CoA + H2O→(S)-3-hydroxytetradecanoyl-CoA (EC 4.2.1.17)
PS417_07030 +0.2
PS417_07575 -0.6
PS417_08535 -0.3
PS417_10680 -0.6
PS417_11445 -2.2
PS417_12870 -0.3
PS417_13835 -0.2
PS417_13845 N.D.
PS417_21195 +0.1
PS417_21215 +0.1
PS417_22625 -0.0
(S)-3-hydroxytetradecanoyl-CoA + NAD+→3-oxomyristoyl-CoA + NADH + H+
PS417_07575 -0.6
PS417_21215 +0.1
Acetyl-CoA C-acyltransferase (in reverse):
3-oxomyristoyl-CoA + coenzyme A→lauroyl-CoA + acetyl-CoA (EC 2.3.1.16)
PS417_06710 -0.4
PS417_07580 N.D.
PS417_10515 -0.2
PS417_13855 +0.3
PS417_15175 N.D.
PS417_19210 +0.1
PS417_21220 -0.3
PS417_03145 -0.4
lauroyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(9E)-dodecenoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes
(9E)-dodecenoyl-CoA + 2 a ferrocytochrome b5 + dioxygen + 2 H+→(8E,10E)-dodeca-8,10-dienoyl-CoA + 2 a ferricytochrome b5 + 2 H2O
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information