MetaCyc Pathway: L-arginine biosynthesis II (acetyl cycle) in Pseudomonas simiae WCS417

Reactions and Genes	R2A with Polymyxin B sulfate 0.003 mg/ml
Glutamate--ammonia ligase: L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
PS417_07365	-0.1
PS417_10580	-1.0
PS417_11785	-0.4
PS417_01655	N.D.
PS417_27135	+0.2
PS417_27140	-0.3
Amino-acid N-acetyltransferase: L-glutamate + acetyl-CoA→N-acetyl-L-glutamate + coenzyme A + H+ (EC 2.3.1.1)
PS417_22535	-3.6
PS417_27195	-3.3
PS417_28175	+0.1
Acetylglutamate kinase: N-acetyl-L-glutamate + ATP→N-acetylglutamyl-phosphate + ADP (EC 2.7.2.8)
PS417_27790	N.D.
Carbamoyl-phosphate synthase (glutamine-hydrolyzing): L-glutamine + 2 ATP + hydrogen carbonate + H2O→L-glutamate + carbamoyl phosphate + 2 ADP + 2 H+ + phosphate (EC 6.3.5.5)
PS417_24040	N.D.
PS417_24045	N.D.
N-acetyl-gamma-glutamyl-phosphate reductase (in reverse): N-acetylglutamyl-phosphate + NADPH + H+→N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.38)
PS417_25660	N.D.
Ornithine carbamoyltransferase: carbamoyl phosphate + L-ornithine→L-citrulline + H+ + phosphate (EC 2.1.3.3)
PS417_05595	-4.2
PS417_22335	+0.4
Acetylornithine transaminase (in reverse): N-acetyl-L-glutamate 5-semialdehyde + L-glutamate→N-acetyl-L-ornithine + 2-oxoglutarate (EC 2.6.1.11)
PS417_08025	-2.5
PS417_21710	-0.8
PS417_25950	-0.5
Argininosuccinate synthase: L-citrulline + ATP + L-aspartate→L-arginino-succinate + AMP + diphosphate + H+ (EC 6.3.4.5)
PS417_05640	N.D.
Argininosuccinate lyase: L-arginino-succinate→L-arginine + fumarate (EC 4.3.2.1)
PS417_21780	+0.6
PS417_27540	N.D.
Glutamate N-acetyltransferase: L-glutamate + N-acetyl-L-ornithine→N-acetyl-L-glutamate + L-ornithine (EC 2.3.1.35)
PS417_22535	-3.6

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information