MetaCyc Pathway: 2-methyl-branched fatty acid β-oxidation in Paraburkholderia sabiae LMG 24235

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Reactions and Genes L-Leucine (C)
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Butyrate--CoA ligase:
a short-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a long-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a long-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.8)
No genes
Butyrate--CoA ligase:
a medium-chain 2-methyl-branched fatty acid + ATP + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + AMP + diphosphate (EC 6.2.1.2)
No genes
a short-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a short-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)
No genes
a medium-chain 2-methyl fatty acyl-CoA + an oxidized electron-transfer flavoprotein + H+→a medium-chain (2E)-2-methyl-2-enoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.7)
QEN71_RS02060 +0.1
QEN71_RS16065 -0.1
QEN71_RS28760 -0.2
Enoyl-CoA hydratase (in reverse):
a long-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a long-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
QEN71_RS01230 +0.1
QEN71_RS02065 +0.0
QEN71_RS02075 -0.2
QEN71_RS04150 -0.1
QEN71_RS05365 -0.0
QEN71_RS06625 -0.2
QEN71_RS08225 N.D.
QEN71_RS10900 -0.0
QEN71_RS12380 -1.1
QEN71_RS12385 -0.6
QEN71_RS15965 +0.1
QEN71_RS16060 +0.2
QEN71_RS19420 -1.4
QEN71_RS19500 -0.2
QEN71_RS21890 +0.2
QEN71_RS22700 +0.2
QEN71_RS24020 +0.2
QEN71_RS24025 -0.1
QEN71_RS26140 -0.3
QEN71_RS27495 +0.2
QEN71_RS27510 +0.2
QEN71_RS29945 +0.0
QEN71_RS30540 +0.7
QEN71_RS30550 N.D.
QEN71_RS30645 +0.4
QEN71_RS32940 -0.2
QEN71_RS33220 -0.1
QEN71_RS35840 -0.1
QEN71_RS38515 N.D.
QEN71_RS39250 -0.1
QEN71_RS39410 -0.1
QEN71_RS39560 +0.2
Enoyl-CoA hydratase (in reverse):
a short-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a short-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
QEN71_RS01230 +0.1
QEN71_RS02065 +0.0
QEN71_RS02075 -0.2
QEN71_RS04150 -0.1
QEN71_RS05365 -0.0
QEN71_RS06625 -0.2
QEN71_RS08225 N.D.
QEN71_RS10900 -0.0
QEN71_RS12380 -1.1
QEN71_RS12385 -0.6
QEN71_RS15965 +0.1
QEN71_RS16060 +0.2
QEN71_RS19420 -1.4
QEN71_RS19500 -0.2
QEN71_RS21890 +0.2
QEN71_RS22700 +0.2
QEN71_RS24020 +0.2
QEN71_RS24025 -0.1
QEN71_RS26140 -0.3
QEN71_RS27495 +0.2
QEN71_RS27510 +0.2
QEN71_RS29945 +0.0
QEN71_RS30540 +0.7
QEN71_RS30550 N.D.
QEN71_RS30645 +0.4
QEN71_RS32940 -0.2
QEN71_RS33220 -0.1
QEN71_RS35840 -0.1
QEN71_RS38515 N.D.
QEN71_RS39250 -0.1
QEN71_RS39410 -0.1
QEN71_RS39560 +0.2
Enoyl-CoA hydratase (in reverse):
a medium-chain (2E)-2-methyl-2-enoyl-CoA + H2O→a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA (EC 4.2.1.17)
QEN71_RS01230 +0.1
QEN71_RS02065 +0.0
QEN71_RS02075 -0.2
QEN71_RS04150 -0.1
QEN71_RS05365 -0.0
QEN71_RS06625 -0.2
QEN71_RS08225 N.D.
QEN71_RS10900 -0.0
QEN71_RS12380 -1.1
QEN71_RS12385 -0.6
QEN71_RS15965 +0.1
QEN71_RS16060 +0.2
QEN71_RS19420 -1.4
QEN71_RS19500 -0.2
QEN71_RS21890 +0.2
QEN71_RS22700 +0.2
QEN71_RS24020 +0.2
QEN71_RS24025 -0.1
QEN71_RS26140 -0.3
QEN71_RS27495 +0.2
QEN71_RS27510 +0.2
QEN71_RS29945 +0.0
QEN71_RS30540 +0.7
QEN71_RS30550 N.D.
QEN71_RS30645 +0.4
QEN71_RS32940 -0.2
QEN71_RS33220 -0.1
QEN71_RS35840 -0.1
QEN71_RS38515 N.D.
QEN71_RS39250 -0.1
QEN71_RS39410 -0.1
QEN71_RS39560 +0.2
3-hydroxyacyl-CoA dehydrogenase:
a long-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a long-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
QEN71_RS02065 +0.0
QEN71_RS21890 +0.2
QEN71_RS24020 +0.2
QEN71_RS29945 +0.0
QEN71_RS37855 N.D.
3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
a short-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a short-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.178)
No genes
3-hydroxyacyl-CoA dehydrogenase:
a medium-chain (3S)-3-hydroxy-2-methylacyl-CoA + NAD+→a medium-chain 2-methyl-3-oxoacyl-CoA + NADH + H+ (EC 1.1.1.35)
QEN71_RS02065 +0.0
QEN71_RS21890 +0.2
QEN71_RS24020 +0.2
QEN71_RS29945 +0.0
QEN71_RS37855 N.D.
Acetyl-CoA C-acyltransferase (in reverse):
a long-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a long-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
QEN71_RS01125 +0.1
QEN71_RS02070 -0.1
QEN71_RS14170 +0.1
QEN71_RS15980 -0.1
QEN71_RS18320 -1.1
QEN71_RS18730 +0.0
QEN71_RS21880 +0.1
QEN71_RS22175 +0.0
QEN71_RS22200 -1.3
QEN71_RS22970 -0.1
QEN71_RS27505 -0.0
Acetyl-CoA C-acyltransferase (in reverse):
a short-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a short-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
QEN71_RS01125 +0.1
QEN71_RS02070 -0.1
QEN71_RS14170 +0.1
QEN71_RS15980 -0.1
QEN71_RS18320 -1.1
QEN71_RS18730 +0.0
QEN71_RS21880 +0.1
QEN71_RS22175 +0.0
QEN71_RS22200 -1.3
QEN71_RS22970 -0.1
QEN71_RS27505 -0.0
Acetyl-CoA C-acyltransferase (in reverse):
a medium-chain 2-methyl-3-oxoacyl-CoA + coenzyme A→a medium-chain 2-methyl fatty acyl-CoA + propanoyl-CoA (EC 2.3.1.16)
QEN71_RS01125 +0.1
QEN71_RS02070 -0.1
QEN71_RS14170 +0.1
QEN71_RS15980 -0.1
QEN71_RS18320 -1.1
QEN71_RS18730 +0.0
QEN71_RS21880 +0.1
QEN71_RS22175 +0.0
QEN71_RS22200 -1.3
QEN71_RS22970 -0.1
QEN71_RS27505 -0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information