MetaCyc Pathway: odd iso-branched-chain fatty acid biosynthesis in Paraburkholderia sabiae LMG 24235

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Reactions and Genes L-Rhamnose (C)
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Branched-chain-amino-acid transaminase:
L-leucine + 2-oxoglutarate→4-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42; 2.6.1.6)
QEN71_RS02410 +0.0
2-oxoisovalerate dehydrogenase (acylating):
4-methyl-2-oxopentanoate + coenzyme A + NAD+→3-methylbutanoyl-CoA + CO2 + NADH (EC 1.2.1.25)
No genes
3-methylbutanoyl-CoA + a malonyl-[acp] + H+→a 5-methyl-3-oxohexanoyl-[acp] + CO2 + coenzyme A (EC 2.3.1.300)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 5-methyl-3-oxohexanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-5-methylhexanoyl-[acp] + NADP+ (EC 1.1.1.100)
QEN71_RS01840 -0.1
QEN71_RS02665 -0.1
QEN71_RS02810 +0.1
QEN71_RS03865 +0.8
QEN71_RS04555 N.D.
QEN71_RS07495 -0.4
QEN71_RS08210 -0.3
QEN71_RS08280 +0.6
QEN71_RS10120 +0.4
QEN71_RS11605 +0.1
QEN71_RS12650 -2.5
QEN71_RS12790 +0.1
QEN71_RS13180 +0.2
QEN71_RS15615 +0.0
QEN71_RS15890 +0.2
QEN71_RS15970 N.D.
QEN71_RS16910 -0.5
QEN71_RS16930 N.D.
QEN71_RS18940 +0.1
QEN71_RS19320 +0.2
QEN71_RS19875 +0.2
QEN71_RS22470 +0.1
QEN71_RS22960 -0.0
QEN71_RS24385 N.D.
QEN71_RS24930 +0.1
QEN71_RS25895 +0.2
QEN71_RS27100 +0.2
QEN71_RS29765 +0.4
QEN71_RS30005 -0.5
QEN71_RS30200 +0.3
QEN71_RS30445 +0.0
QEN71_RS31020 +0.2
QEN71_RS31395 +0.2
QEN71_RS32355 +0.1
QEN71_RS32915 +0.0
QEN71_RS33390 -0.1
QEN71_RS33420 +0.0
QEN71_RS33735 +0.1
QEN71_RS33860 N.D.
QEN71_RS34325 +0.4
QEN71_RS34445 -0.7
QEN71_RS35630 -0.1
QEN71_RS36135 N.D.
QEN71_RS36540 -0.1
QEN71_RS36910 -0.2
QEN71_RS37725 -0.0
QEN71_RS38560 -0.1
QEN71_RS38920 -0.1
QEN71_RS39090 +0.4
QEN71_RS39175 -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-5-methylhexanoyl-[acp]→a (2E)-5-methylhex-2-enoyl-[acp] + H2O (EC 4.2.1.59)
QEN71_RS06475 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADH + H+→a 5-methylhexanoyl-[acp] + NAD+ (EC 1.3.1.9)
QEN71_RS06165 N.D.
QEN71_RS13540 +0.2
QEN71_RS30430 +0.6
QEN71_RS30525 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-5-methylhex-2-enoyl-[acp] + NADPH + H+→a 5-methylhexanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
QEN71_RS10120 +0.4
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 5-methylhexanoyl-[acp] + a malonyl-[acp] + H+→a 7-methyl-3-oxooctanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
QEN71_RS03870 +0.4
QEN71_RS04565 N.D.
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 7-methyl-3-oxooctanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-7-methyloctanoyl-[acp] + NADP+ (EC 1.1.1.100)
QEN71_RS01840 -0.1
QEN71_RS02665 -0.1
QEN71_RS02810 +0.1
QEN71_RS03865 +0.8
QEN71_RS04555 N.D.
QEN71_RS07495 -0.4
QEN71_RS08210 -0.3
QEN71_RS08280 +0.6
QEN71_RS10120 +0.4
QEN71_RS11605 +0.1
QEN71_RS12650 -2.5
QEN71_RS12790 +0.1
QEN71_RS13180 +0.2
QEN71_RS15615 +0.0
QEN71_RS15890 +0.2
QEN71_RS15970 N.D.
QEN71_RS16910 -0.5
QEN71_RS16930 N.D.
QEN71_RS18940 +0.1
QEN71_RS19320 +0.2
QEN71_RS19875 +0.2
QEN71_RS22470 +0.1
QEN71_RS22960 -0.0
QEN71_RS24385 N.D.
QEN71_RS24930 +0.1
QEN71_RS25895 +0.2
QEN71_RS27100 +0.2
QEN71_RS29765 +0.4
QEN71_RS30005 -0.5
QEN71_RS30200 +0.3
QEN71_RS30445 +0.0
QEN71_RS31020 +0.2
QEN71_RS31395 +0.2
QEN71_RS32355 +0.1
QEN71_RS32915 +0.0
QEN71_RS33390 -0.1
QEN71_RS33420 +0.0
QEN71_RS33735 +0.1
QEN71_RS33860 N.D.
QEN71_RS34325 +0.4
QEN71_RS34445 -0.7
QEN71_RS35630 -0.1
QEN71_RS36135 N.D.
QEN71_RS36540 -0.1
QEN71_RS36910 -0.2
QEN71_RS37725 -0.0
QEN71_RS38560 -0.1
QEN71_RS38920 -0.1
QEN71_RS39090 +0.4
QEN71_RS39175 -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-7-methyloctanoyl-[acp]→a (2E)-7-methyloct-2-enoyl-[acp] + H2O (EC 4.2.1.59)
QEN71_RS06475 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADH + H+→a 7-methyloctanoyl-[acp] + NAD+ (EC 1.3.1.9)
QEN71_RS06165 N.D.
QEN71_RS13540 +0.2
QEN71_RS30430 +0.6
QEN71_RS30525 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-7-methyloct-2-enoyl-[acp] + NADPH + H+→a 7-methyloctanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
QEN71_RS10120 +0.4
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 7-methyloctanoyl-[acp] + a malonyl-[acp] + H+→a 9-methyl-3-oxodecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
QEN71_RS03870 +0.4
QEN71_RS04565 N.D.
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 9-methyl-3-oxodecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-9-methyldecanoyl-[acp] + NADP+ (EC 1.1.1.100)
QEN71_RS01840 -0.1
QEN71_RS02665 -0.1
QEN71_RS02810 +0.1
QEN71_RS03865 +0.8
QEN71_RS04555 N.D.
QEN71_RS07495 -0.4
QEN71_RS08210 -0.3
QEN71_RS08280 +0.6
QEN71_RS10120 +0.4
QEN71_RS11605 +0.1
QEN71_RS12650 -2.5
QEN71_RS12790 +0.1
QEN71_RS13180 +0.2
QEN71_RS15615 +0.0
QEN71_RS15890 +0.2
QEN71_RS15970 N.D.
QEN71_RS16910 -0.5
QEN71_RS16930 N.D.
QEN71_RS18940 +0.1
QEN71_RS19320 +0.2
QEN71_RS19875 +0.2
QEN71_RS22470 +0.1
QEN71_RS22960 -0.0
QEN71_RS24385 N.D.
QEN71_RS24930 +0.1
QEN71_RS25895 +0.2
QEN71_RS27100 +0.2
QEN71_RS29765 +0.4
QEN71_RS30005 -0.5
QEN71_RS30200 +0.3
QEN71_RS30445 +0.0
QEN71_RS31020 +0.2
QEN71_RS31395 +0.2
QEN71_RS32355 +0.1
QEN71_RS32915 +0.0
QEN71_RS33390 -0.1
QEN71_RS33420 +0.0
QEN71_RS33735 +0.1
QEN71_RS33860 N.D.
QEN71_RS34325 +0.4
QEN71_RS34445 -0.7
QEN71_RS35630 -0.1
QEN71_RS36135 N.D.
QEN71_RS36540 -0.1
QEN71_RS36910 -0.2
QEN71_RS37725 -0.0
QEN71_RS38560 -0.1
QEN71_RS38920 -0.1
QEN71_RS39090 +0.4
QEN71_RS39175 -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-9-methyldecanoyl-[acp]→a (2E)-9-methyldec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
QEN71_RS06475 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADH + H+→a 9-methyldecanoyl-[acyl-carrier protein] + NAD+ (EC 1.3.1.9)
QEN71_RS06165 N.D.
QEN71_RS13540 +0.2
QEN71_RS30430 +0.6
QEN71_RS30525 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-9-methyldec-2-enoyl-[acp] + NADPH + H+→a 9-methyldecanoyl-[acyl-carrier protein] + NADP+ (EC 1.3.1.10; 1.3.1.104)
QEN71_RS10120 +0.4
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 9-methyldecanoyl-[acyl-carrier protein] + a malonyl-[acp] + H+→a 11-methyl-3-oxododecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
QEN71_RS03870 +0.4
QEN71_RS04565 N.D.
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 11-methyl-3-oxododecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-11-methyldodecanoyl-[acp] + NADP+ (EC 1.1.1.100)
QEN71_RS01840 -0.1
QEN71_RS02665 -0.1
QEN71_RS02810 +0.1
QEN71_RS03865 +0.8
QEN71_RS04555 N.D.
QEN71_RS07495 -0.4
QEN71_RS08210 -0.3
QEN71_RS08280 +0.6
QEN71_RS10120 +0.4
QEN71_RS11605 +0.1
QEN71_RS12650 -2.5
QEN71_RS12790 +0.1
QEN71_RS13180 +0.2
QEN71_RS15615 +0.0
QEN71_RS15890 +0.2
QEN71_RS15970 N.D.
QEN71_RS16910 -0.5
QEN71_RS16930 N.D.
QEN71_RS18940 +0.1
QEN71_RS19320 +0.2
QEN71_RS19875 +0.2
QEN71_RS22470 +0.1
QEN71_RS22960 -0.0
QEN71_RS24385 N.D.
QEN71_RS24930 +0.1
QEN71_RS25895 +0.2
QEN71_RS27100 +0.2
QEN71_RS29765 +0.4
QEN71_RS30005 -0.5
QEN71_RS30200 +0.3
QEN71_RS30445 +0.0
QEN71_RS31020 +0.2
QEN71_RS31395 +0.2
QEN71_RS32355 +0.1
QEN71_RS32915 +0.0
QEN71_RS33390 -0.1
QEN71_RS33420 +0.0
QEN71_RS33735 +0.1
QEN71_RS33860 N.D.
QEN71_RS34325 +0.4
QEN71_RS34445 -0.7
QEN71_RS35630 -0.1
QEN71_RS36135 N.D.
QEN71_RS36540 -0.1
QEN71_RS36910 -0.2
QEN71_RS37725 -0.0
QEN71_RS38560 -0.1
QEN71_RS38920 -0.1
QEN71_RS39090 +0.4
QEN71_RS39175 -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-11-methyldodecanoyl-[acp]→a (2E)-11-methyldodec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
QEN71_RS06475 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADH + H+→an 11-methyldodecanoyl-[acp] + NAD+ (EC 1.3.1.9)
QEN71_RS06165 N.D.
QEN71_RS13540 +0.2
QEN71_RS30430 +0.6
QEN71_RS30525 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-11-methyldodec-2-enoyl-[acp] + NADPH + H+→an 11-methyldodecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
QEN71_RS10120 +0.4
Beta-ketoacyl-acyl-carrier-protein synthase II:
an 11-methyldodecanoyl-[acp] + a malonyl-[acp] + H+→a 13-methyl-3-oxotetradecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
QEN71_RS03870 +0.4
QEN71_RS04565 N.D.
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 13-methyl-3-oxotetradecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp] + NADP+ (EC 1.1.1.100)
QEN71_RS01840 -0.1
QEN71_RS02665 -0.1
QEN71_RS02810 +0.1
QEN71_RS03865 +0.8
QEN71_RS04555 N.D.
QEN71_RS07495 -0.4
QEN71_RS08210 -0.3
QEN71_RS08280 +0.6
QEN71_RS10120 +0.4
QEN71_RS11605 +0.1
QEN71_RS12650 -2.5
QEN71_RS12790 +0.1
QEN71_RS13180 +0.2
QEN71_RS15615 +0.0
QEN71_RS15890 +0.2
QEN71_RS15970 N.D.
QEN71_RS16910 -0.5
QEN71_RS16930 N.D.
QEN71_RS18940 +0.1
QEN71_RS19320 +0.2
QEN71_RS19875 +0.2
QEN71_RS22470 +0.1
QEN71_RS22960 -0.0
QEN71_RS24385 N.D.
QEN71_RS24930 +0.1
QEN71_RS25895 +0.2
QEN71_RS27100 +0.2
QEN71_RS29765 +0.4
QEN71_RS30005 -0.5
QEN71_RS30200 +0.3
QEN71_RS30445 +0.0
QEN71_RS31020 +0.2
QEN71_RS31395 +0.2
QEN71_RS32355 +0.1
QEN71_RS32915 +0.0
QEN71_RS33390 -0.1
QEN71_RS33420 +0.0
QEN71_RS33735 +0.1
QEN71_RS33860 N.D.
QEN71_RS34325 +0.4
QEN71_RS34445 -0.7
QEN71_RS35630 -0.1
QEN71_RS36135 N.D.
QEN71_RS36540 -0.1
QEN71_RS36910 -0.2
QEN71_RS37725 -0.0
QEN71_RS38560 -0.1
QEN71_RS38920 -0.1
QEN71_RS39090 +0.4
QEN71_RS39175 -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-13-methyltetradecanoyl-[acp]→a (2E)-13-methyltetradec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
QEN71_RS06475 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADH + H+→a 13-methyltetradecanoyl-[acp] + NAD+ (EC 1.3.1.9)
QEN71_RS06165 N.D.
QEN71_RS13540 +0.2
QEN71_RS30430 +0.6
QEN71_RS30525 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-13-methyltetradec-2-enoyl-[acp] + NADPH + H+→a 13-methyltetradecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)
QEN71_RS10120 +0.4
Beta-ketoacyl-acyl-carrier-protein synthase II:
a 13-methyltetradecanoyl-[acp] + a malonyl-[acp] + H+→a 15-methyl-3-oxohexadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO2 (EC 2.3.1.179)
QEN71_RS03870 +0.4
QEN71_RS04565 N.D.
Oleoyl-[acyl-carrier-protein] hydrolase:
a 13-methyltetradecanoyl-[acp] + H2O→13-methyltetradecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes
3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 15-methyl-3-oxohexadecanoyl-[acp] + NADPH + H+→a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp] + NADP+ (EC 1.1.1.100)
QEN71_RS01840 -0.1
QEN71_RS02665 -0.1
QEN71_RS02810 +0.1
QEN71_RS03865 +0.8
QEN71_RS04555 N.D.
QEN71_RS07495 -0.4
QEN71_RS08210 -0.3
QEN71_RS08280 +0.6
QEN71_RS10120 +0.4
QEN71_RS11605 +0.1
QEN71_RS12650 -2.5
QEN71_RS12790 +0.1
QEN71_RS13180 +0.2
QEN71_RS15615 +0.0
QEN71_RS15890 +0.2
QEN71_RS15970 N.D.
QEN71_RS16910 -0.5
QEN71_RS16930 N.D.
QEN71_RS18940 +0.1
QEN71_RS19320 +0.2
QEN71_RS19875 +0.2
QEN71_RS22470 +0.1
QEN71_RS22960 -0.0
QEN71_RS24385 N.D.
QEN71_RS24930 +0.1
QEN71_RS25895 +0.2
QEN71_RS27100 +0.2
QEN71_RS29765 +0.4
QEN71_RS30005 -0.5
QEN71_RS30200 +0.3
QEN71_RS30445 +0.0
QEN71_RS31020 +0.2
QEN71_RS31395 +0.2
QEN71_RS32355 +0.1
QEN71_RS32915 +0.0
QEN71_RS33390 -0.1
QEN71_RS33420 +0.0
QEN71_RS33735 +0.1
QEN71_RS33860 N.D.
QEN71_RS34325 +0.4
QEN71_RS34445 -0.7
QEN71_RS35630 -0.1
QEN71_RS36135 N.D.
QEN71_RS36540 -0.1
QEN71_RS36910 -0.2
QEN71_RS37725 -0.0
QEN71_RS38560 -0.1
QEN71_RS38920 -0.1
QEN71_RS39090 +0.4
QEN71_RS39175 -0.4
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-15-methylhexadecanoyl-[acp]→a (2E)-15-methylhexadec-2-enoyl-[acp] + H2O (EC 4.2.1.59)
QEN71_RS06475 N.D.
Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADH + H+→a 15-methylhexadecanoyl-[acp] + NAD+ (EC 1.3.1.9)
QEN71_RS06165 N.D.
QEN71_RS13540 +0.2
QEN71_RS30430 +0.6
QEN71_RS30525 +0.1
Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-15-methylhexadec-2-enoyl-[acp] + NADPH + H+→a 15-methylhexadecanoyl-[acp] + NADP+ (EC 1.3.1.10; 1.3.1.104)
QEN71_RS10120 +0.4
Oleoyl-[acyl-carrier-protein] hydrolase:
a 15-methylhexadecanoyl-[acp] + H2O→15-methylhexadecanoate + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.14)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information