MetaCyc Pathway: photosynthetic 3-hydroxybutanoate biosynthesis (engineered) in Paraburkholderia sabiae LMG 24235

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Reactions and Genes D-Sorbitol (C)
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2 H2O[in] + hν[cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + hν[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out] (EC 1.18.1.2)
QEN71_RS00515 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H+→D-glyceraldehyde 3-phosphate + NADP+ + phosphate (EC 1.2.1.13)
No genes
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)
QEN71_RS24165 -2.2
QEN71_RS41515 +0.4
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)
QEN71_RS02435 N.D.
QEN71_RS13015 +0.1
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate (EC 4.1.2.13)
QEN71_RS02435 N.D.
QEN71_RS13015 +0.1
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
QEN71_RS03770 N.D.
QEN71_RS32775 -0.2
QEN71_RS34115 +0.1
Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H2O→D-sedoheptulose 7-phosphate + phosphate (EC 3.1.3.37)
No genes
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)
QEN71_RS11540 +0.0
QEN71_RS11590 +0.1
QEN71_RS11595 -0.1
QEN71_RS27010 N.D.
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)
QEN71_RS11540 +0.0
QEN71_RS11590 +0.1
QEN71_RS11595 -0.1
QEN71_RS27010 N.D.
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)
QEN71_RS22905 N.D.
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)
QEN71_RS27540 N.D.
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+ (EC 2.7.1.19)
QEN71_RS32780 +0.2
QEN71_RS34120 -0.1
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+ (EC 4.1.1.39)
QEN71_RS16030 +0.2
QEN71_RS32760 -0.0
QEN71_RS32765 +0.4
QEN71_RS38380 -0.1
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
QEN71_RS01445 -5.2
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)
QEN71_RS02425 -4.7
Phosphopyruvate hydratase:
2-phospho-D-glycerate→H2O + phosphoenolpyruvate (EC 4.2.1.11)
QEN71_RS05880 N.D.
QEN71_RS41510 +0.3
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
QEN71_RS02430 -1.5
QEN71_RS12195 -0.0
QEN71_RS41440 -0.1
pyruvate + coenzyme A + NAD+→CO2 + acetyl-CoA + NADH (EC 1.2.1.104)
QEN71_RS05730 N.D.
QEN71_RS23700 -0.1
QEN71_RS40215 -0.2
QEN71_RS40225 -0.1
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
QEN71_RS01125 +0.1
QEN71_RS02070 -0.3
QEN71_RS14170 -0.3
QEN71_RS15980 -0.6
QEN71_RS18320 -0.8
QEN71_RS18730 -0.5
QEN71_RS21880 +0.6
QEN71_RS22175 +0.0
QEN71_RS22200 -1.1
QEN71_RS22970 -0.2
QEN71_RS27505 +0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
QEN71_RS02065 -0.1
QEN71_RS21890 +0.1
QEN71_RS24020 -0.1
QEN71_RS29945 -0.2
QEN71_RS37855 N.D.
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+ (EC 1.1.1.36)
QEN71_RS22195 -1.0
QEN71_RS32115 -0.1
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
No genes
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information