MetaCyc Pathway: L-glutamate and L-glutamine biosynthesis in Escherichia coli BW25113

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Reactions and Genes Putrescine (N)
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Glutamate dehydrogenase (NAD(P)(+)) (in reverse):
2-oxoglutarate + ammonium + NAD(P)H + H+→L-glutamate + NAD(P)+ + H2O (EC 1.4.1.3)
No genes
Glutamate synthase (ferredoxin) (in reverse):
2-oxoglutarate + L-glutamine + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2 L-glutamate + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.4.7.1)
No genes
Glutamate synthase (NADH) (in reverse):
2-oxoglutarate + L-glutamine + NADH + H+→2 L-glutamate + NAD+ (EC 1.4.1.14)
b3213 (gltD) -3.4
Glutamate synthase (NADPH):
L-glutamine + H2O→L-glutamate + ammonia + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
b0032 (carA) -5.8
b0033 (carB) -5.2
b0485 (ybaS) -0.1
b0674 (asnB) -5.5
b1263 (trpD) -2.7
b1264 (trpE) -1.7
b1524 (yneH) +0.0
b1812 (pabB) -0.2
b2468 (aegA) +0.1
b2507 (guaA) N.D.
b2611 (ypjD) -0.8
b2780 (pyrG) N.D.
b2887 (ygfT) +0.0
b3212 (gltB) -3.4
b3213 (gltD) -3.4
b3360 (pabA) +0.0
Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
b3870 (glnA) -2.9
Glutamate dehydrogenase (NADP(+)) (in reverse):
2-oxoglutarate + ammonium + NADPH + H+→L-glutamate + NADP+ + H2O (EC 1.4.1.4)
b1761 (gdhA) -0.2
b3213 (gltD) -3.4
Glutamate synthase (NADPH) (in reverse):
2-oxoglutarate + ammonia + NADPH + 2 H+→L-glutamate + NADP+ + H2O (EC 1.4.1.13; 1.4.1.14)
b2468 (aegA) +0.1
b2611 (ypjD) -0.8
b2887 (ygfT) +0.0
b3212 (gltB) -3.4
b3213 (gltD) -3.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information