MetaCyc Pathway: superpathway of L-methionine biosynthesis (by sulfhydrylation) in Escherichia coli BW25113

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Reactions and Genes Putrescine (N)
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
b0928 (aspC) -2.9
b4340 (yjiR) +0.1
Sulfate adenylyltransferase:
sulfate + ATP + H+→adenosine 5'-phosphosulfate + diphosphate (EC 2.7.7.4)
b2751 (cysN) -3.0
b2752 (cysD) -3.7
Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H+ (EC 2.7.1.25)
b2750 (cysC) -4.7
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
b0002 (thrA) -4.6
b3940 (metL) -1.0
b4024 (lysC) +1.3
Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H+ (EC 1.8.4.8)
b2762 (cysH) -4.3
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
b2319 (usg) -0.3
b3433 (asd) N.D.
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
b0002 (thrA) -4.6
b3940 (metL) -1.0
Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H+→hydrogen sulfide + 3 NADP+ + 3 H2O (EC 1.8.1.2)
b2763 (cysI) -3.6
b2764 (cysJ) -4.2
Homoserine O-acetyltransferase:
L-homoserine + acetyl-CoA→O-acetyl-L-homoserine + coenzyme A (EC 2.3.1.31)
No genes
O-acetylhomoserine aminocarboxypropyltransferase:
O-acetyl-L-homoserine + hydrogen sulfide→L-homocysteine + acetate + H+ (EC 2.5.1.49)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
b3829 (metE) -4.2
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
b4019 (metH) -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information