MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Serratia liquefaciens MT49

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Reactions and Genes Cobalt chloride 60 uM, anaerobic
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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
IAI46_03290 +0.1
IAI46_03295 -0.1
IAI46_03375 +0.1
IAI46_03380 +0.2
IAI46_17615 +0.2
IAI46_18380 +0.0
IAI46_21055 -0.0
IAI46_24355 +0.1
IAI46_24360 +0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
IAI46_24325 +0.1
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
IAI46_03155 +0.0
IAI46_24345 +0.0
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
IAI46_02655 -0.1
IAI46_07885 +0.1
IAI46_12310 -0.5
IAI46_14220 -1.8
IAI46_19495 -0.3
IAI46_21740 +0.0
IAI46_22730 -0.0
IAI46_22910 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information