MetaCyc Pathway: toluene degradation I (aerobic) (via o-cresol) in Serratia liquefaciens MT49

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Reactions and Genes Cobalt chloride 60 uM, anaerobic
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toluene + NADH + dioxygen + H+→2-methylphenol + NAD+ + H2O (EC 1.14.13.243)
No genes
2-methylphenol + NADH + dioxygen + H+→3-methylcatechol + NAD+ + H2O (EC 1.14.13.243)
No genes
Catechol 2,3-dioxygenase:
3-methylcatechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H+ (EC 1.13.11.2)
IAI46_12470 +0.2
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + acetate + H+ (EC 3.7.1.25)
No genes
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate (EC 4.2.1.80)
IAI46_05780 -0.2
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate (EC 4.1.3.39)
IAI46_05770 -0.1
IAI46_16105 +0.2
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10)
IAI46_05775 +0.2
IAI46_14220 -1.8
IAI46_16110 -0.3

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information