MetaCyc Pathway: hexitol fermentation to lactate, formate, ethanol and acetate in Serratia liquefaciens MT49

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Reactions and Genes Cobalt chloride 60 uM, anaerobic
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Mannitol-1-phosphate 5-dehydrogenase:
D-mannitol 1-phosphate + NAD+→β-D-fructofuranose 6-phosphate + NADH + H+ (EC 1.1.1.17)
IAI46_00160 -0.4
Sorbitol-6-phosphate 2-dehydrogenase:
D-sorbitol 6-phosphate + NAD+→keto-D-fructose 6-phosphate + NADH + H+ (EC 1.1.1.140)
IAI46_19070 +0.0
6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H+ (EC 2.7.1.11)
IAI46_11575 -0.1
IAI46_24645 -2.2
Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)
IAI46_04740 -0.0
IAI46_10085 -0.1
IAI46_19050 +0.1
IAI46_21005 N.D.
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)
IAI46_01795 +0.0
IAI46_06500 -0.1
IAI46_07775 +0.2
IAI46_24605 -0.0
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
IAI46_23990 -0.0
IAI46_24635 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
IAI46_14355 N.D.
IAI46_16675 +0.2
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
IAI46_21010 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
IAI46_03000 +0.2
IAI46_06410 N.D.
IAI46_24710 -0.2
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)
IAI46_06410 N.D.
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
IAI46_03615 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
IAI46_11185 +0.1
IAI46_14585 +0.1
L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(S)-lactate + NAD+ (EC 1.1.1.27)
No genes
Pyruvate, water dikinase:
pyruvate + ATP + H2O→phosphoenolpyruvate + AMP + 2 H+ + phosphate (EC 2.7.9.2)
IAI46_11115 -0.1
Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)
IAI46_08605 -1.7
IAI46_08610 -2.1
IAI46_13885 +0.1
IAI46_13890 +0.2
IAI46_19615 +0.1
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
IAI46_05775 +0.2
IAI46_14220 -1.8
IAI46_16110 -0.3
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
IAI46_17755 -1.5
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
IAI46_14770 +0.3
IAI46_17750 -1.6
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
IAI46_02655 -0.1
IAI46_07885 +0.1
IAI46_12310 -0.5
IAI46_14220 -1.8
IAI46_19495 -0.3
IAI46_21740 +0.0
IAI46_22730 -0.0
IAI46_22910 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information