MetaCyc Pathway: glutaminyl-tRNAgln biosynthesis via transamidation in Escherichia coli BW25113
| Reactions and Genes | D-Alanine (N) |
|---|---|
| remove | |
| Glutamate synthase (NADPH): L-glutamine + H2O→ammonia + L-glutamate + H+ (EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7) | |
| b0032 (carA) | -3.3 |
| b0033 (carB) | -4.0 |
| b0485 (ybaS) | +0.1 |
| b0674 (asnB) | -1.4 |
| b1263 (trpD) | -2.7 |
| b1264 (trpE) | -2.6 |
| b1524 (yneH) | +0.2 |
| b1812 (pabB) | +0.1 |
| b2468 (aegA) | +0.0 |
| b2507 (guaA) | N.D. |
| b2611 (ypjD) | -1.0 |
| b2780 (pyrG) | N.D. |
| b2887 (ygfT) | -0.1 |
| b3212 (gltB) | -4.1 |
| b3213 (gltD) | -4.2 |
| b3360 (pabA) | +0.1 |
| Glutamate--tRNA(Gln) ligase: L-glutamate + ATP + a tRNAGln→an L-glutamyl-[tRNAGln] + AMP + diphosphate (EC 6.1.1.24) | |
| No genes | |
| Glutaminyl-tRNA synthase (glutamine-hydrolyzing): an L-glutamyl-[tRNAGln] + ATP→a 5-phosphooxy-L-glutamyl-[tRNAGln] + ADP (EC 6.3.5.7) | |
| No genes | |
| Glutaminyl-tRNA synthase (glutamine-hydrolyzing): a 5-phosphooxy-L-glutamyl-[tRNAGln] + ammonia→an L-glutaminyl-[tRNAGln] + phosphate (EC 6.3.5.7) | |
| No genes | |
Links:
- Pathway details at MetaCyc
- Fitness data for 16 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information