MetaCyc Pathway: pyruvate fermentation to hexanol (engineered) in Pseudomonas putida KT2440

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Reactions and Genes 4-Hydroxyvalerate (C) (40mM)
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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PP_0582 +0.2
PP_1377 +0.0
PP_2051 +0.0
PP_2137 -0.4
PP_2215 -0.1
PP_3280 -0.4
PP_3355 -0.1
PP_3754 -3.1
PP_4636 -0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
PP_2047 +0.6
PP_2136 -0.4
PP_3755 -3.4
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
PP_1412 -0.1
PP_2047 +0.6
PP_2136 -0.4
PP_3726 +0.1
PP_3732 -0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
PP_1893 +0.7
PP_2039 +0.2
PP_2216 +0.0
PP_2793 -4.4
PP_3226 -0.3
PP_3638 -0.1
PP_3725 -0.0
PP_4635 N.D.
Acetyl-CoA C-acyltransferase:
acetyl-CoA + butanoyl-CoA→3-oxohexanoyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
PP_0582 +0.2
PP_1377 +0.0
PP_2051 +0.0
PP_2137 -0.4
PP_2215 -0.1
PP_3280 -0.4
PP_3355 -0.1
PP_3754 -3.1
PP_4636 -0.1
3-hydroxyacyl-CoA dehydrogenase (in reverse):
3-oxohexanoyl-CoA + NADH + H+→(S)-3-hydroxyhexanoyl-CoA + NAD+ (EC 1.1.1.35)
PP_2047 +0.6
PP_2136 -0.4
Long-chain-enoyl-CoA hydratase:
(S)-3-hydroxyhexanoyl-CoA→(2E)-hex-2-enoyl-CoA + H2O (EC 4.2.1.74)
PP_1412 -0.1
PP_2047 +0.6
PP_2136 -0.4
PP_3726 +0.1
PP_3732 -0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
(2E)-hex-2-enoyl-CoA + NADH + H+→hexanoyl-CoA + NAD+ (EC 1.3.1.44)
PP_4635 N.D.
hexanoyl-CoA + NAD(P)H + H+→1-hexanal + coenzyme A + NAD(P)+
No genes
1-hexanal + NADH + H+→hexan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information