MetaCyc Pathway: L-valine biosynthesis in Escherichia coli BW25113
| Reactions and Genes | L-Glutamine (N) |
|---|---|
| remove | |
| Acetolactate synthase: 2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6) | |
| b0078 (ilvH) | -0.3 |
| b3670 (ilvN) | -1.8 |
| b3671 (ilvB) | -3.1 |
| b3769 (ilvM) | +0.5 |
| b0077 (ilvI) | -0.4 |
| Ketol-acid reductoisomerase (in reverse): (S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86) | |
| b3774 (ilvC) | -5.1 |
| Dihydroxy-acid dehydratase: (2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9) | |
| b0269 (yagF) | +0.1 |
| b4297 (yjhG) | +0.1 |
| b3771 (ilvD) | -4.9 |
| Branched-chain-amino-acid transaminase (in reverse): 3-methyl-2-oxobutanoate + L-glutamate→L-valine + 2-oxoglutarate (EC 2.6.1.42) | |
| b3770 (ilvE) | -5.0 |
Links:
- Pathway details at MetaCyc
- Fitness data for 10 genes
- All MetaCyc pathways for Escherichia coli BW25113
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information