MetaCyc Pathway: L-asparagine biosynthesis III (tRNA-dependent) in Escherichia coli BW25113

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Reactions and Genes L-Aspartic Acid (N)
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Glutamate synthase (NADPH):
L-glutamine + H2O→ammonia + L-glutamate + H+
(EC 1.4.1.13; 1.4.1.14; 1.4.7.1; 2.6.1.85; 3.5.1.2; 3.5.1.38; 4.1.3.27; 4.3.2.10; 4.3.3.6; 6.3.4.2; 6.3.5.13; 6.3.5.2; 6.3.5.4; 6.3.5.5; 6.3.5.6; 6.3.5.7)
b0032 (carA) -5.2
b0033 (carB) -4.6
b0485 (ybaS) -0.0
b0674 (asnB) -1.7
b1263 (trpD) -2.7
b1264 (trpE) -2.2
b1524 (yneH) -0.9
b1812 (pabB) -0.4
b2468 (aegA) +0.1
b2507 (guaA) N.D.
b2611 (ypjD) -0.4
b2780 (pyrG) N.D.
b2887 (ygfT) +0.2
b3212 (gltB) +0.2
b3213 (gltD) +0.2
b3360 (pabA) +0.1
Aspartate--tRNA(Asn) ligase:
L-aspartate + tRNAAsn + ATP→an L-aspartyl-[tRNAAsn] + AMP + diphosphate
(EC 6.1.1.23)
No genes
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
an L-aspartyl-[tRNAAsn] + ATP→a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ADP
(EC 6.3.5.6)
No genes
Asparaginyl-tRNA synthase (glutamine-hydrolyzing):
a 4-phosphooxy-L-aspartyl-[tRNAAsn] + ammonia→an L-asparaginyl-[tRNAAsn] + phosphate
(EC 6.3.5.6)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information